Immune cell profiles in synovial fluid after anterior cruciate ligament and meniscus injuries.
Immune cell profiles in synovial fluid after anterior cruciate ligament and meniscus injuries.
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DOI：
10.1186/s13075-021-02661-1
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发表时间：
2021-11-04
影响因子：
4.9
通讯作者：
McNulty AL
中科院分区：
文献类型：
--
作者：
Kim-Wang SY;Holt AG;McGowan AM;Danyluk ST;Goode AP;Lau BC;Toth AP;Wittstein JR;DeFrate LE;Yi JS;McNulty AL
前十字韧带和半月板撕裂是常见的膝关节损伤。尽管这些损伤后创伤性骨关节炎(PTOA)的发生率很高，但其致病因素仍不清楚。在这项研究中，我们研究了前交叉韧带和半月板手术时正常和损伤关节的免疫细胞图谱。29例患者(14例半月板损伤和15例前交叉韧带损伤)接受了前交叉韧带和/或半月板手术，但对侧膝关节正常。手术过程中，从正常和受伤的膝关节抽出滑液。将滑液细胞制粒、洗涤，并用由细胞表面蛋白荧光抗体组成的抗体鸡尾酒进行染色。用多色流式细胞术分析滑液中的免疫细胞。在前10名受试者中使用了广谱免疫细胞仪表板。根据这些结果，在随后的19名受试者中使用了T细胞特异性小组。使用广谱免疫细胞板，我们在受伤的膝关节中检测到比配对的正常膝关节明显更多的总活细胞和CD3T细胞。此外，T细胞超过500细胞阈值的受伤膝盖明显更多。在损伤的膝关节内，CD4和CD8T细胞能够分化成亚群。不同损伤类型间的总CD4T细胞频率差异有统计学意义，但不同损伤类型间的CD4T细胞亚群和CD8T细胞亚群之间差异无统计学意义。我们的发现提供了基础数据，表明前交叉韧带和半月板损伤诱导了一个免疫细胞丰富的微环境，该微环境主要由具有多种T辅助表型的T细胞组成。未来研究免疫细胞与关节退行性变之间的关系可能会加深对关节损伤后PTOA的病理生理学的理解。
Anterior cruciate ligament (ACL) and meniscus tears are common knee injuries. Despite the high rate of post-traumatic osteoarthritis (PTOA) following these injuries, the contributing factors remain unclear. In this study, we characterized the immune cell profiles of normal and injured joints at the time of ACL and meniscal surgeries. Twenty-nine patients (14 meniscus-injured and 15 ACL-injured) undergoing ACL and/or meniscus surgery but with a normal contralateral knee were recruited. During surgery, synovial fluid was aspirated from both normal and injured knees. Synovial fluid cells were pelleted, washed, and stained with an antibody cocktail consisting of fluorescent antibodies for cell surface proteins. Analysis of immune cells in the synovial fluid was performed by polychromatic flow cytometry. A broad spectrum immune cell panel was used in the first 10 subjects. Based on these results, a T cell-specific panel was used in the subsequent 19 subjects. Using the broad spectrum immune cell panel, we detected significantly more total viable cells and CD3 T cells in the injured compared to the paired normal knees. In addition, there were significantly more injured knees with T cells above a 500-cell threshold. Within the injured knees, CD4 and CD8 T cells were able to be differentiated into subsets. The frequency of total CD4 T cells was significantly different among injury types, but no statistical differences were detected among CD4 and CD8 T cell subsets by injury type. Our findings provide foundational data showing that ACL and meniscus injuries induce an immune cell-rich microenvironment that consists primarily of T cells with multiple T helper phenotypes. Future studies investigating the relationship between immune cells and joint degeneration may provide an enhanced understanding of the pathophysiology of PTOA following joint injury.
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影响因子：
3.8
作者：
Hayward, A. L.;Deehan, D. J.;Sutherland, A. G.
通讯作者：
Sutherland, A. G.
影响因子：
4.8
作者：
Clair, Andrew J.;Kingery, Matthew T.;Strauss, Eric J.
通讯作者：
Strauss, Eric J.
影响因子：
9.1
作者：
Cho, Bon-A;Sim, Ji Hyun;Kim, Hang-Rae
通讯作者：
Kim, Hang-Rae
影响因子：
2.5
作者：
Fuhrmann, Inga Katharina;Steinhagen, Joern;Schumacher, Udo
通讯作者：
Schumacher, Udo
DOI：
10.1097/01.blo.0000197079.26600.09
发表时间：
2006-01-01
影响因子：
4.2
作者：
Andriacchi, Thomas P.;Briant, Paul L.;Koo, Seungbum
通讯作者：
Koo, Seungbum