A Rotifer-Based Technique to Rear Zebrafish Larvae in Small Academic Settings

A Rotifer-Based Technique to Rear Zebrafish Larvae in Small Academic Settings
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在小型学术环境中饲养斑马鱼幼虫的基于轮虫的技术

DOI:
10.1089/zeb.2015.1182
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发表时间:
2016
期刊:
影响因子:
2
通讯作者:
Sisson, Barbara E.
Sisson, Barbara E.
中科院分区:
生物学4区
文献类型:
--
作者:
Allen, Raymond L.;Wallace, Robert L.;Sisson, Barbara E.

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将斑马鱼从幼虫养大到幼鱼是很费力的,需要频繁的换水和不断地培养活饲料。对于小型机构来说,这项任务变得更加困难,因为它们没有必要的资金、设备或人员来维护通常用于斑马鱼养殖的大型系统。为了向较小的机构开放这一机会,开发了一种成本效益高的方案来培养嗜盐的浮游轮虫——柔顺臂轮虫;然后,这些轮虫被用来将斑马鱼幼崽饲养到幼崽。通过使用这些方法,小型机构可以轻松地以经济有效的方式饲养斑马鱼胚胎,而无需建立广泛的养鱼设施。此外,培养轮虫提供了一种微后生动物,作为教学和本科生研究各种主题的模式生物,包括衰老,毒理学和捕食者-猎物动力学。
Raising zebrafish from larvae to juveniles can be laborious, requiring frequent water exchanges and continuous culturing of live feed. This task becomes even more difficult for small institutions that do not have access to the necessary funding, equipment, or personnel to maintain large-scale systems usually employed in zebrafish husbandry. To open this opportunity to smaller institutions, a cost-efficient protocol was developed to cultureNannochloropsisto feed the halophilic, planktonic rotiferBrachionus plicatilis; the rotifers were then used to raise larval zebrafish to juveniles. By using these methods, small institutions can easily raise zebrafish embryos in a cost-efficient manner without the need to establish an extensive fish-raising facility. In addition, culturing rotifers provides a micrometazoan that serves as a model organism for teaching and undergraduate research studies for a variety of topics, including aging, toxicology, and predator–prey dynamics.
课堂上的斑马鱼
DOI: --
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