Analysis of human gliomas by swab touch spray-mass spectrometry: applications to intraoperative assessment of surgical margins and presence of oncometabolites.
Analysis of human gliomas by swab touch spray-mass spectrometry: applications to intraoperative assessment of surgical margins and presence of oncometabolites.
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DOI：
10.1039/c7an01334e
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发表时间：
2017-10-23
期刊：
影响因子：
--
通讯作者：
Cooks RG
中科院分区：
文献类型：
--
作者：
Pirro V;Llor RS;Jarmusch AK;Alfaro CM;Cohen-Gadol AA;Hattab EM;Cooks RG
使用医用拭子的接触喷雾质谱法是一种环境电离技术（在开放空气中电离未处理的样品），在快速识别组织的疾病状态和更好地表征切除边缘方面具有潜在的术中应用。为了探索这一潜力，我们研究了29例人类脑肿瘤标本，并获得了证据表明该技术可以提供与脑癌相关的诊断分子信息。使用医用拭子的接触喷雾涉及使用医用拭子在几mm 2的空间尺度上对组织进行物理采样，随后在添加溶剂和施加高电压时直接从拭子尖端发生电离。使用乙腈、N，N-二甲基甲酰胺和乙醇的三级混合物，从组织中提取膜衍生的磷脂和钉螺，将其掺入喷雾的微滴中，抽真空进入质谱仪，并在所得质谱中进行表征。根据质谱中的复合磷脂模式以及通过测量N-乙酰基-天冬氨酸单独评估肿瘤细胞负荷。异柠檬酸脱氢酶基因的突变状态通过检测癌代谢产物2-羟基戊二酸来确定。缺乏样品预处理使得使用医用拭子的接触喷雾质谱法成为快速手术评估的可行术中策略。
Touch spray mass spectrometry using medical swabs is an ambient ionization technique (ionization of unprocessed sample in the open air) that has potential intraoperative application in quickly identifying the disease state of tissue and in better characterizing the resection margin. To explore this potential, we studied 29 human brain tumor specimens and obtained evidence that this technique can provide diagnostic molecular information that is relevant to brain cancer. Touch spray using medical swabs involves the physical sampling of tissue using a medical swab on a spatial scale of a few mm2 with subsequent ionization occurring directly from the swab tip upon addition of solvent and application of a high voltage. Using a tertiary mixture of acetonitrile, N,N-dimethylformamide, and ethanol, membrane-derived phospholipids and oncometabolites are extracted from the tissue, incorporated into the sprayed microdroplets, vacuumed into the mass spectrometer, and characterized in the resulting mass spectra. The tumor cell load was assessed from the complex phospholipid pattern in the mass spectra and also separately by measurement of N-acetyl-aspartate. Mutation status of the isocitrate dehydrogenase gene was determined via detection of the oncometabolite 2-hydroxyglutarate. The lack of sample pretreatment makes touch spray mass spectrometry using medical swabs a feasible intraoperative strategy for rapid surgical assessment.
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触摸喷雾质谱法，用于对复杂样品的原位分析。
DOI：
10.1039/c4an00548a
发表时间：
2014-06-07
期刊：
The Analyst
影响因子：
--
作者：
Kerian KS;Jarmusch AK;Cooks RG
通讯作者：
Cooks RG
影响因子：
7.4
作者：
Gomez-Rios, German Augusto;Liu, Chang;Pawliszyn, Janusz
通讯作者：
Pawliszyn, Janusz
在增强胶质母细胞瘤的增强和非增强部分的肿瘤浸润：与组织病理学的相关性。
影响因子：
3.7
作者：
Eidel O;Burth S;Neumann JO;Kieslich PJ;Sahm F;Jungk C;Kickingereder P;Bickelhaupt S;Mundiyanapurath S;Bäumer P;Wick W;Schlemmer HP;Kiening K;Unterberg A;Bendszus M;Radbruch A
通讯作者：
Radbruch A
影响因子：
17.1
作者：
Balog, Julia;Sasi-Szabo, Laszlo;Takats, Zoltan
通讯作者：
Takats, Zoltan
影响因子：
3.2
作者：
Eberlin, Livia S.;Ferreira, Christina R.;Dill, Allison L.;Ifa, Demian R.;Cheng, Liang;Cooks, R. Graham
通讯作者：
Cooks, R. Graham