Nondestructive, histologically compatible tissue imaging by desorption electrospray ionization mass spectrometry.
Nondestructive, histologically compatible tissue imaging by desorption electrospray ionization mass spectrometry.
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DOI：
10.1002/cbic.201100411
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发表时间：
2011-09-19
期刊：
影响因子：
3.2
通讯作者：
Cooks, R. Graham
中科院分区：
文献类型：
--
作者：
Eberlin, Livia S.;Ferreira, Christina R.;Dill, Allison L.;Ifa, Demian R.;Cheng, Liang;Cooks, R. Graham
关键词：
成像质谱学是一种强有力的技术，在许多科学领域得到了越来越多的关注和广泛的应用。利用它提供的关于动植物组织内多种内源和外源分子分布的综合信息，正在进行许多生物学应用[1]。在成像MS技术[2]中，那些基于环境电离的技术[3]，例如解吸电喷雾电离质谱仪(DESI-MS)[4]、激光烧蚀电喷雾电离(LAESI)[5]和大气压飞秒激光成像质谱仪(AP fs-LDI IMS)[6][7]，允许在大气压下进行分析，而无需大量的样品制备。在过去的几年里，许多工作都在推进环境成像质谱学，特别是在癌症诊断方面[8]。通过将化学信息添加到形态显微镜分析中来提高组织病理学癌症评估的准确性的前景现在代表着一种可实现的进步。许多研究证明了Desi-MS成像作为诊断工具的能力，在这些研究中，直接从不同类型的组织，如肾[9]、膀胱癌[10]、前列腺癌[11]和脑癌[12]检测到的脂谱能够诊断多种类型的人类癌症。然而，技术挑战仍然存在，验证研究仍然需要成功地将显微镜和质谱学信息合并到常规组织病理学工作流程中。到目前为止，组织学和DESI-MS之间的相关性是通过将获得的离子图像与连续切片的病理评估诊断进行比较来实现的，通常是苏木精-伊红染色切片[9]。尽管这一策略对于常规显微镜下的光学图像评估是足够的，但明确的相关性要求使用相同的组织切片进行形态和MS成像评估[13]。Desi-MS成像与组织化学检查不兼容的主要原因是最常用的溶剂系统，如甲醇/水和乙腈/水1：1(v/v)，产生广泛的化学信息，但在过程中完全破坏自然组织形态，
Imaging mass spectrometry (MS) is a powerful technique which has been gaining increased attention and broad use in many fields of science. Many biological applications are being pursued using the comprehensive information it provides on the distribution of multiple endogenous and exogenous molecules within animal and plant tissues [1]. Amongst the imaging MS techniques [2], those based on ambient ionization [3] such as desorption electrospray ionization mass spectrometry (DESI-MS)[4], laser ablation electrospray ionization (LAESI)[5] and atmospheric pressure fentosecond laser imaging mass spectrometry (AP fs-LDI IMS)[6] amongst others [7], allow analysis at atmospheric pressure without significant sample preparation. In the past few years, much effort has gone into advancing ambient imaging mass spectrometry, especially in cancer diagnostics [8]. The prospect of improving the accuracy of histopathological cancer evaluation by adding chemical information to morphological microscopic analysis now represents an attainable advance.The capabilities of DESI-MS imaging as a diagnostic tool are demonstrated in many studies, in which the lipid profiles directly detected from different types of tissues, such as kidney [9], bladder [10], prostate [11], and brain [12] cancers enabled diagnosis of multiple types of human cancers. However, technical challenges remain and validation studies are still needed to successfully merge microscopic and mass spectrometric information into routine histopathology workflow. Correlation between histology and DESI-MS has thus far been performed by comparing the ion images obtained to the diagnosis from pathological evaluation of a serial section, typically a hematoxylin and eosin (H&E) stained section [9]. Even though this strategy is sufficient for optical image evaluation under routine microscopic pathology, unambiguous correlation demands the use of the same tissue section for morphological and MS imaging evaluation [13]. The primary reason for incompatibility of DESI-MS imaging with histochemical examination is that the most commonly used solvent systems, such as methanol/water and acetonitrile/water 1: 1 (v/v), yield extensive chemical information but completely destroy the native tissue morphology in the process,
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