Change in the developmental fate of the chick optic vesicle from the neural retina to the telencephalon
Change in the developmental fate of the chick optic vesicle from the neural retina to the telencephalon
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雏鸡视泡从神经视网膜到端脑发育命运的变化
作者：
Shirahama;Misaki;Steinfeld;Karaiwa;Taketani;Shigeru;Vogel-Höpker;Paul G;Masasuke
前脑发育成端脑、间脑和视泡（OV）。OV进一步发育成视杯，其内层和外层分别发育成神经视网膜和视网膜色素上皮（RPE）。我们通过胚胎移植和外植体培养的方法研究了OV命运的变化。将从10体节期鸡胚切除的OV从周围组织（表面外胚层和间充质）中分离出来，并移植回其在宿主胚胎中的原始位置。神经视网膜特异性基因的表达，如Rax和Vsx 2（Chx 10），在移植物中下调。相反，端脑特异性基因Emx 1的表达出现在移植物的近端区域，并且在靠近表皮的移植物的远端部分，观察到RPE特异性基因Mitf的表达。外植体培养研究表明，当单独培养OVs时，Rax持续表达，而与周围组织（间充质和表皮）无关。当没有周围组织的OV与前脑紧密接触培养时，Rax表达在外植体中下调，而Emx 1表达上调。这些发现表明，第10阶段的鸡卵母细胞在其发育命运方面是双电位的，无论是神经视网膜还是端脑，周围组织在其实际命运中具有关键作用。体外组织培养模型表明，在前脑和/或其周围组织的影响下，OV改变其从视网膜到端脑的命运。
The forebrain develops into the telencephalon, diencephalon, and optic vesicle (OV). The OV further develops into the optic cup, the inner and outer layers of which develop into the neural retina and retinal pigmented epithelium (RPE), respectively. We studied the change in fate of the OV by using embryonic transplantation and explant culture methods. OVs excised from 10‐somite stage chick embryos were freed from surrounding tissues (the surface ectoderm and mesenchyme) and were transplanted back to their original position in host embryos. Expression of neural retina‐specific genes, such asRaxandVsx2(Chx10), was downregulated in the transplants. Instead, expression of the telencephalon‐specific geneEmx1emerged in the proximal region of the transplants, and in the distal part of the transplants close to the epidermis, expression of an RPE‐specific geneMitfwas observed. Explant culture studies showed that when OVs were cultured alone,Raxwas continuously expressed regardless of surrounding tissues (mesenchyme and epidermis). When OVs without surrounding tissues were cultured in close contact with the anterior forebrain,Raxexpression became downregulated in the explants, andEmx1expression became upregulated. These findings indicate that chick OVs at stage 10 are bi‐potential with respect to their developmental fates, either for the neural retina or for the telencephalon, and that the surrounding tissues have a pivotal role in their actual fates. An in vitro tissue culture model suggests that under the influence of the anterior forebrain and/or its surrounding tissues, the OV changes its fate from the retina to the telencephalon.
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影响因子：
2.6
作者：
Briata, P;DiBlas, E;Corte, G
通讯作者：
Corte, G
Chx10 充当控制原始视网膜区域特征的分子途径的调节剂。
影响因子：
2.7
作者：
Zi Wang;S. Yasugi;Y. Ishii
通讯作者：
Y. Ishii
眼外背侧信号影响视神经泡的发育命运并形成视神经上皮的模式。
DOI：
--
发表时间：
2005
期刊：
Develop. Growth Differe. 47
影响因子：
--
作者：
Kagiyama Y;Gotouda K;Sakagami J;Yasuda K;Mochii M and Araki M
通讯作者：
Mochii M and Araki M
FGF8 通过对 Otx2 表达的抑制作用诱导异位峡部组织者的形成和峡部小脑的发育。
DOI：
10.1242/dev.126.6.1189
发表时间：
1999
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Martinez,S;Crossley,PH;Cobos,I;Rubenstein,JL;Martin,GR
通讯作者：
Martin,GR
影响因子：
16.2
作者：
MARTINEZ, S;WASSEF, M;ALVARADOMALLART, RM
通讯作者：
ALVARADOMALLART, RM