Molecular pain markers correlate with pH-sensitive MRI signal in a pig model of disc degeneration.
Molecular pain markers correlate with pH-sensitive MRI signal in a pig model of disc degeneration.
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DOI：
10.1038/s41598-018-34582-6
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发表时间：
2018-11-26
影响因子：
4.6
通讯作者：
Gazit D
中科院分区：
文献类型：
--
作者：
Bez M;Zhou Z;Sheyn D;Tawackoli W;Giaconi JC;Shapiro G;Ben David S;Gazit Z;Pelled G;Li D;Gazit D
椎间盘（IVD）退变是慢性腰痛的主要原因，每年影响数百万人。然而，导致这种疼痛的特定IVD的识别是基于定性的视觉解释，而不是客观的结果。诊断疼痛相关IVD的一种可能方法是识别酸性IVD，因为已经假设IVD内的pH值降低介导椎间盘源性疼痛。我们假设定量化学交换饱和转移（qCEST）MRI可以检测IVD的pH值变化，从而用于客观和非侵入性地诊断病理性疼痛IVD。为了检验这一假设，使用尤卡坦小型猪IVD变性的手术模型。对退化IVD内pH值的直接测量显示退化后pH值显著下降，这与qCEST信号显著增加相关。对收获的退化IVD的基因分析显示，IVD退化后疼痛、神经和炎症相关标志物显著上调。在疼痛标志物的表达和qCEST信号的增加之间观察到强正相关。总的来说，这些研究结果表明，这种方法可以用来确定哪种IVD引起腰痛，从而为疼痛和手术治疗提供有价值的指导。
Intervertebral disc (IVD) degeneration is a leading cause of chronic low back pain that affects millions of people every year. Yet identification of the specific IVD causing this pain is based on qualitative visual interpretation rather than objective findings. One possible approach to diagnosing pain-associated IVD could be to identify acidic IVDs, as decreased pH within an IVD has been postulated to mediate discogenic pain. We hypothesized that quantitative chemical exchange saturation transfer (qCEST) MRI could detect pH changes in IVDs, and thence be used to diagnose pathologically painful IVDs objectively and noninvasively. To test this hypothesis, a surgical model of IVD degeneration in Yucatan minipigs was used. Direct measurement of pH inside the degenerated IVDs revealed a significant drop in pH after degeneration, which correlated with a significant increase in the qCEST signal. Gene analysis of harvested degenerated IVDs revealed significant upregulation of pain-, nerve- and inflammatory-related markers after IVD degeneration. A strong positive correlation was observed between the expression of pain markers and the increase in the qCEST signal. Collectively, these findings suggest that this approach might be used to identify which IVD is causing low back pain, thereby providing valuable guidance for pain and surgical management.
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影响因子：
2.1
作者：
Kang, Chang Ho;Kim, Yun Hwan;Sung, Deuk Jae
通讯作者：
Sung, Deuk Jae
影响因子：
3.7
作者：
Lefevre-Colau, Marie-Martine;Fayad, Fouad;Poiraudeau, Serge
通讯作者：
Poiraudeau, Serge
影响因子：
168.9
作者：
Freemont, AJ;Peacock, TE;Jayson, MIV
通讯作者：
Jayson, MIV
椎间盘低 pH 值与腰痛之间的关系：系统评价。
DOI：
10.5114/aoms.2012.32401
发表时间：
2012-12-20
期刊：
Archives of medical science : AMS
影响因子：
--
作者：
Liang CZ;Li H;Tao YQ;Zhou XP;Yang ZR;Li FC;Chen QX
通讯作者：
Chen QX
影响因子：
5.9
作者：
Haneder, Stefan;Apprich, Sebastian R.;Trattnig, Siegfried
通讯作者：
Trattnig, Siegfried