Cell death stages in single apoptotic and necrotic cells monitored by Raman microspectroscopy.
Cell death stages in single apoptotic and necrotic cells monitored by Raman microspectroscopy.
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DOI：
10.1038/srep04698
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发表时间：
2014-04-15
影响因子：
4.6
通讯作者：
Schenke-Layland K
中科院分区：
文献类型：
--
作者：
Brauchle E;Thude S;Brucker SY;Schenke-Layland K
虽然细胞凋亡和坏死有不同的特点，但在体外识别和区分凋亡和坏死细胞死亡是具有挑战性的。免疫细胞学和生化分析是目前监测细胞死亡途径的金标准；然而，这些标准分析是侵入性的，导致大量细胞死亡，并阻碍持续监测实验。在这项研究中，利用拉曼光谱分析了室温(RT)诱导的单个Saos-2和SW-1353细胞的凋亡和热触发的坏死。基于拉曼光谱和荧光显微镜的结合，有助于对明确的细胞死亡方式进行有针对性的分析，包括早期和晚期的细胞凋亡以及坏死。在这两个细胞系中发现了光谱漂移，这些变化反映了RT诱导的细胞凋亡或热介导的坏死所特有的生化变化。基于单细胞拉曼光谱的监督分类模型指定了细胞的凋亡性和坏死性死亡。总之，拉曼光谱可以非侵入性地、连续地监测细胞死亡，这可能有助于揭示复杂的病理生理或药物诱导的细胞死亡过程。
Although apoptosis and necrosis have distinct features, the identification and discrimination of apoptotic and necrotic cell death in vitro is challenging. Immunocytological and biochemical assays represent the current gold standard for monitoring cell death pathways; however, these standard assays are invasive, render large numbers of cells and impede continuous monitoring experiments. In this study, both room temperature (RT)-induced apoptosis and heat-triggered necrosis were analyzed in individual Saos-2 and SW-1353 cells by utilizing Raman microspectroscopy. A targeted analysis of defined cell death modalities, including early and late apoptosis as well as necrosis, was facilitated based on the combination of Raman spectroscopy with fluorescence microscopy. Spectral shifts were identified in the two cell lines that reflect biochemical changes specific for either RT-induced apoptosis or heat-mediated necrosis. A supervised classification model specified apoptotic and necrotic cell death based on single cell Raman spectra. To conclude, Raman spectroscopy allows a non-invasive, continuous monitoring of cell death, which may help shedding new light on complex pathophysiological or drug-induced cell death processes.
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影响因子：
2.8
作者：
Pudlas, Marieke;Brauchle, Eva;Schenke-Layland, Katja
通讯作者：
Schenke-Layland, Katja
影响因子：
12.4
作者：
Johnson, C. E.;Freel, C. D.;Kornbluth, S.
通讯作者：
Kornbluth, S.
影响因子：
4.2
作者：
Bonnier, F.;Byrne, H. J.
通讯作者：
Byrne, H. J.
影响因子：
2.5
作者：
Fenn, Michael B.;Pappu, Vijay;Pardalos, Panos M.
通讯作者：
Pardalos, Panos M.
DOI：
10.1007/978-1-62703-383-1_1
发表时间：
2013-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Janko, Christina;Munoz, Luis;Herrmann, Martin
通讯作者：
Herrmann, Martin