Epigenetic variation in mangrove plants occurring in contrasting natural environment.
Epigenetic variation in mangrove plants occurring in contrasting natural environment.
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DOI：
10.1371/journal.pone.0010326
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发表时间：
2010-04-26
期刊：
影响因子：
3.7
通讯作者：
Ferreira PC
中科院分区：
文献类型：
--
作者：
Lira-Medeiros CF;Parisod C;Fernandes RA;Mata CS;Cardoso MA;Ferreira PC
表观遗传修饰，如胞嘧啶甲基化，在植物物种中是遗传的，可能会对生物或非生物胁迫做出反应，在不改变基因组序列的情况下影响基因表达。总状花环藻是一种红树林植物，生活在自然环境中，每天都会受到盐分和营养变化的影响，从而导致形态差异。这项工作旨在揭示CpG甲基化变异是如何在来自不同环境压力的两个邻近栖息地(河边(RS)或盐沼(SM))的个体之间分布的，以及这种变异是如何与观察到的形态变异相关联的。RS和SM植株在树高、直径、叶宽和叶面积等形态性状上存在显著差异，导致SM植株植株较小，叶片较小。利用甲基敏感扩增多态性(MSAP)分析了这些群体的总状花序基因组的遗传和表观遗传(CpG-甲基化)变异。与RS(32.1%的基因座有甲基化样本)相比，SM植株是低甲基化的(14.6%的基因座有甲基化样本)。在这两个地点，表观遗传的种群内多样性显著大于遗传数据，但SM的表观遗传多样性也低于RS。基于频率的(GST)和多变量(βST)估计种群结构的方法显示，表观遗传数据在不同地点之间的差异性明显大于遗传数据。协惯性分析，联合探索遗传和表观遗传数据，表明具有相似遗传图谱的个体呈现出不同的表观遗传图谱，这是特定环境中群体的特征，表明CpG甲基化变化可能与环境异质性有关。盐沼和河滨总状花序的个体虽然在形态上存在明显的差异，但遗传分化很少，但DNA甲基化分化丰富，这表明自然植物种群的表观遗传变异在帮助个体适应不同的环境方面具有重要作用。
Epigenetic modifications, such as cytosine methylation, are inherited in plant species and may occur in response to biotic or abiotic stress, affecting gene expression without changing genome sequence. Laguncularia racemosa, a mangrove species, occurs in naturally contrasting habitats where it is subjected daily to salinity and nutrient variations leading to morphological differences. This work aims at unraveling how CpG-methylation variation is distributed among individuals from two nearby habitats, at a riverside (RS) or near a salt marsh (SM), with different environmental pressures and how this variation is correlated with the observed morphological variation. Significant differences were observed in morphological traits such as tree height, tree diameter, leaf width and leaf area between plants from RS and SM locations, resulting in smaller plants and smaller leaf size in SM plants. Methyl-Sensitive Amplified Polymorphism (MSAP) was used to assess genetic and epigenetic (CpG-methylation) variation in L. racemosa genomes from these populations. SM plants were hypomethylated (14.6% of loci had methylated samples) in comparison to RS (32.1% of loci had methylated samples). Within-population diversity was significantly greater for epigenetic than genetic data in both locations, but SM also had less epigenetic diversity than RS. Frequency-based (GST) and multivariate (βST) methods that estimate population structure showed significantly greater differentiation among locations for epigenetic than genetic data. Co-Inertia analysis, exploring jointly the genetic and epigenetic data, showed that individuals with similar genetic profiles presented divergent epigenetic profiles that were characteristic of the population in a particular environment, suggesting that CpG-methylation changes may be associated with environmental heterogeneity. In spite of significant morphological dissimilarities, individuals of L. racemosa from salt marsh and riverside presented little genetic but abundant DNA methylation differentiation, suggesting that epigenetic variation in natural plant populations has an important role in helping individuals to cope with different environments.
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影响因子：
4.9
作者：
Cardoso, MA;Provan, J;De Oliveira, DE
通讯作者：
De Oliveira, DE
影响因子：
3.1
作者：
Keyte, Anna L.;Percifield, Ryan;Wendel, Jonathan F.
通讯作者：
Wendel, Jonathan F.
影响因子：
3.1
作者：
Cervera, MT;Ruiz-García, L;Martínez-Zapater, JM
通讯作者：
Martínez-Zapater, JM
影响因子：
3.1
作者：
Jaligot, E;Beulé, T;Rival, A
通讯作者：
Rival, A
DOI：
10.1590/s0001-37652006000200004
发表时间：
2006-06-01
期刊：
Anais da Academia Brasileira de Ciências
影响因子：
--
作者：
Barth, Ortrud M.;São-Thiago, Luiz E.U.;Barros, Marcia A.
通讯作者：
Barros, Marcia A.