Whole Genome Sequencing for Determining the Source of Mycobacterium bovis Infections in Livestock Herds and Wildlife in New Zealand.
Whole Genome Sequencing for Determining the Source of Mycobacterium bovis Infections in Livestock Herds and Wildlife in New Zealand.
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DOI：
10.3389/fvets.2018.00272
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发表时间：
2018
影响因子：
3.2
通讯作者：
Collins DM
中科院分区：
文献类型：
--
作者：
Price-Carter M;Brauning R;de Lisle GW;Livingstone P;Neill M;Sinclair J;Paterson B;Atkinson G;Knowles G;Crews K;Crispell J;Kao R;Robbe-Austerman S;Stuber T;Parkhill J;Wood J;Harris S;Collins DM
牛分枝杆菌分离物DNA指纹鉴定的能力有助于确定野生动物在新西兰牛结核病持续存在中的作用。DNA指纹鉴定结果目前有助于指导野生动物控制措施，也有助于追踪牲畜流动造成的感染源。在过去的5年中，我们已经发展了通过比较全基因组测序（WGS）牛分枝杆菌样本的序列来区分新西兰（NZ）牛分枝杆菌分离株的能力。WGS提供了比其他已建立的分型方法更高的分辨率，并大大改善了新西兰牛分枝杆菌类型区域定位的定义。描述了三次暴发调查，结果表明WGS分析如何导致确认感染的流行病学来源，通过排除其他可能的来源更好地定义新的感染源，并在被认为没有受感染野生动物的地区发现可能的野生动物感染。常规使用WGS分析来寻找新的牛支原体感染将是根除新西兰牲畜和野生动物牛结核病战略的重要组成部分。
The ability to DNA fingerprint Mycobacterium bovis isolates helped to define the role of wildlife in the persistence of bovine tuberculosis in New Zealand. DNA fingerprinting results currently help to guide wildlife control measures and also aid in tracing the source of infections that result from movement of livestock. During the last 5 years we have developed the ability to distinguish New Zealand (NZ) M. bovis isolates by comparing the sequences of whole genome sequenced (WGS) M. bovis samples. WGS provides much higher resolution than our other established typing methods and greatly improves the definition of the regional localization of NZ M. bovis types. Three outbreak investigations are described and results demonstrate how WGS analysis has led to the confirmation of epidemiological sourcing of infection, to better definition of new sources of infection by ruling out other possible sources, and has revealed probable wildlife infection in an area considered to be free of infected wildlife. The routine use of WGS analyses for sourcing new M. bovis infections will be an important component of the strategy employed to eradicate bovine TB from NZ livestock and wildlife.
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影响因子：
6.7
作者：
Biek, Roman;O'Hare, Anthony;Kao, Rowland R.
通讯作者：
Kao, Rowland R.
通过分枝杆菌亚种的整个基因组测序鉴定的串联重复键入的局限性。国家和牛群的旁结核病。
影响因子：
4.4
作者：
Ahlstrom C;Barkema HW;Stevenson K;Zadoks RN;Biek R;Kao R;Trewby H;Haupstein D;Kelton DF;Fecteau G;Labrecque O;Keefe GP;McKenna SL;De Buck J
通讯作者：
De Buck J
影响因子：
3.3
作者：
Collins, Desmond M.
通讯作者：
Collins, Desmond M.
DOI：
10.2460/javma.251.2.206
发表时间：
2017-07-15
影响因子：
1.9
作者：
Bruning-Fann, Colleen S.;Robbe-Austerman, Suelee;Marks, David
通讯作者：
Marks, David
影响因子：
1.4
作者：
Binney, B. M.;Biggs, P. J.;French, N. P.
通讯作者：
French, N. P.