Resistance of Arabidopsis thaliana to the green peach aphid, Myzus persicae, involves camalexin and is regulated by microRNAs.
Resistance of Arabidopsis thaliana to the green peach aphid, Myzus persicae, involves camalexin and is regulated by microRNAs.
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DOI：
10.1111/nph.12218
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发表时间：
2013-06
期刊：
影响因子：
--
通讯作者：
Hogenhout SA
中科院分区：
文献类型：
--
作者：
Kettles GJ;Drurey C;Schoonbeek HJ;Maule AJ;Hogenhout SA
小分子RNA在植物抗病毒、抗病原菌和食叶昆虫等复杂反应中起着重要作用。我们研究了小RNA途径是否参与了拟南芥对韧皮部取食昆虫--绿色桃蚜（Myzus persicae）的抗性。我们使用了2周的繁殖力测定来评估蚜虫对拟南芥RNA沉默和防御途径突变体的表现。实时定量聚合酶链反应被用来监测不同蚜虫易感性植物中防御相关基因的转录活性。采用高效液相色谱-质谱法测量抗微生物化合物camalexin的积累。人工饲料测定允许评估Camalexin对蚜虫性能的影响。桃蚜在拟南芥microRNA（miRNA）途径突变体上产生显著更少的后代。不能加工miRNA的植物对蚜虫侵染的反应是增加植物抗毒素缺乏3（PAD 3）的诱导和camalexin的产生。蚜虫在以拟南芥为食时摄取Camalexin，并且在Camalexin产生缺陷的pad 3和cyp 79 b2/cyp 79 b3突变体上更成功。蚜虫在含有Camalexin的人工饲料中产生较少的后代。我们的数据表明，camalexin功能超出抗菌防御，也包括半翅目昆虫。这项工作还强调了广泛的作用，miRNA介导的调节与半翅目害虫的抗性相关的次级代谢防御途径。
Small RNAs play important roles in resistance to plant viruses and the complex responses against pathogens and leaf-chewing insects. We investigated whether small RNA pathways are involved in Arabidopsis resistance against a phloem-feeding insect, the green peach aphid (Myzus persicae). We used a 2-wk fecundity assay to assess aphid performance on Arabidopsis RNA silencing and defence pathway mutants. Quantitative real-time polymerase chain reaction was used to monitor the transcriptional activity of defence-related genes in plants of varying aphid susceptibility. High-performance liquid chromatography-mass spectrometry was employed to measure the accumulation of the antimicrobial compound camalexin. Artificial diet assays allowed the assessment of the effect of camalexin on aphid performance. Myzus persicae produces significantly less progeny on Arabidopsis microRNA (miRNA) pathway mutants. Plants unable to process miRNAs respond to aphid infestation with increased induction of PHYTOALEXIN DEFICIENT3 (PAD3) and production of camalexin. Aphids ingest camalexin when feeding on Arabidopsis and are more successful on pad3 and cyp79b2/cyp79b3 mutants defective in camalexin production. Aphids produce less progeny on artificial diets containing camalexin. Our data indicate that camalexin functions beyond antimicrobial defence to also include hemipteran insects. This work also highlights the extensive role of the miRNA-mediated regulation of secondary metabolic defence pathways with relevance to resistance against a hemipteran pest.
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