MUC1 selectively targets human pancreatic cancer in orthotopic nude mouse models.
MUC1 selectively targets human pancreatic cancer in orthotopic nude mouse models.
复制标题
DOI：
10.1371/journal.pone.0122100
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Bouvet M
中科院分区：
文献类型：
--
作者：
Park JY;Hiroshima Y;Lee JY;Maawy AA;Hoffman RM;Bouvet M
本研究的目的是确定MUC 1抗体与荧光团结合是否可用于显示胰腺癌。抗MUC 1（CT 2）抗体与550 nm或650 nm荧光团缀合。采用裸鼠建立胰腺癌皮下和原位模型。Western blot和流式细胞术分析证实MUC 1在人胰腺癌细胞系BxPC-3和Panc-1中表达。用荧光团缀合的抗MUC 1抗体的免疫细胞化学显示Panc-1癌细胞膜上的荧光区域。通过尾静脉注射缀合的抗MUC 1抗体后，皮下移植的Panc-1和BxPC-3肿瘤发出强荧光信号。在皮下肿瘤模型中，在肿瘤边缘和细胞之间的空间周围注意到来自缀合的抗MUC 1抗体的荧光信号。缀合的抗MUC 1抗体与原位移植的Panc-1和BxPC-3模型中的肿瘤结合，使肿瘤能够成像。这项研究表明，荧光团标记的抗MUC 1抗体可以在体外和体内显示胰腺肿瘤，并可能有助于改善胰腺癌的诊断和治疗。
The goal of this study was to determine whether MUC1 antibody conjugated with a fluorophore could be used to visualize pancreatic cancer. Anti-MUC1 (CT2) antibody was conjugated with 550 nm or 650 nm fluorophores. Nude mouse were used to make subcutaneous and orthotopic models of pancreatic cancer. Western blot and flow cytometric analysis confirmed the expression of MUC1 in human pancreatic cancer cell lines including BxPC-3 and Panc-1. Immunocytochemistry with fluorophore conjugated anti-MUC1 antibody demonstrated fluorescent areas on the membrane of Panc-1 cancer cells. After injecting the conjugated anti-MUC1 antibodies via the tail vein, subcutaneously transplanted Panc-1 and BxPC-3 tumors emitted strong fluorescent signals. In the subcutaneous tumor models, the fluorescent signal from the conjugated anti-MUC1 antibody was noted around the margin of the tumor and space between the cells. The conjugated anti-MUC1 antibody bound the tumor in orthotopically-transplanted Panc-1 and BxPC-3 models enabling the tumors to be imaged. This study showed that fluorophore conjugated anti-MUC1 antibodies could visualize pancreatic tumors in vitro and in vivo and may help to improve the diagnosis and treatment of pancreatic cancer.
登录
查看更多内容
DOI：
10.1073/pnas.89.12.5645
发表时间：
1992-06-15
影响因子：
11.1
作者：
FU, XY;GUADAGNI, F;HOFFMAN, RM
通讯作者：
HOFFMAN, RM
影响因子：
6.4
作者：
LIEBER, M;MAZZETTA, J;TODARO, G
通讯作者：
TODARO, G
影响因子：
3.5
作者：
Maawy, Ali A.;Hiroshima, Yukihiko;Bouvet, Michael
通讯作者：
Bouvet, Michael
DOI：
10.1186/bcr2409
发表时间：
2009
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Pegram MD;Borges VF;Ibrahim N;Fuloria J;Shapiro C;Perez S;Wang K;Schaedli Stark F;Courtenay Luck N
通讯作者：
Courtenay Luck N
影响因子：
6.4
作者：
Medarova, Z;Pham, W;Moore, A
通讯作者：
Moore, A