Effects of temporal hydrostatic pressure on tissue-engineered bovine articular cartilage constructs.
Effects of temporal hydrostatic pressure on tissue-engineered bovine articular cartilage constructs.
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DOI：
10.1089/ten.tea.2008.0200
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发表时间：
2009-05
影响因子：
--
通讯作者：
Athanasiou KA
中科院分区：
文献类型：
--
作者：
Elder BD;Athanasiou KA
本研究的目的是确定时间静水压力(HP)对无支架牛关节软骨构建物性能的影响。这项研究分为三个阶段：首先，确定了适合HP应用的控制措施。其次，在三个不同的时间点(6-10天、10-14天和14-18天)应用10兆帕的静态高压，以确定结构开发中应用HP最有利的窗口。第三，在第二阶段中确定的10-14天的静态HP应用的时间效应在2、4和8周被评估。评估了压缩和拉伸机械性能、GAG和胶原含量、GAG和胶原的组织学以及I型和II型胶原的免疫组织化学。当在第一阶段确定的培养对照在第二阶段使用时，从10天到14天的HP应用导致聚集体模数显著增加1.4倍，同时伴随着GAG含量的增加，而在所有时间点应用HP都提高了拉伸性能和胶原含量。在第三阶段，HP对GAG含量、胶原含量和压缩硬度有直接影响，而拉伸硬度有延迟增加。在8周时仍有增强的抗拉硬度。这项研究首次考察了HP对生物力学性能的即时和长期影响，并证明了HP在构建过程中有一个最佳的应用时间。这些发现是令人兴奋的，因为HP刺激可以形成健壮的组织工程软骨；例如，在10 Mpa的静态HP刺激下，4周时的聚集体弹性系数为273±123kpa，杨氏弹性系数为1.6±0.4 Mpa，Gag/湿重为6.1±1.4%，胶原/湿重为10.6±2.4%。
The objective of this study was to determine the effects of temporal hydrostatic pressure (HP) on the properties of scaffoldless bovine articular cartilage constructs. The study was organized in three phases: First, a suitable control for HP application was identified. Second, 10 MPa static HP was applied at three different timepoints (6–10 days, 10–14 days, and 14–18 days) to identify a window in construct development when HP application would be most beneficial. Third, the temporal effects of 10–14-day static HP application, as determined in phase II, were assessed at 2, 4, and 8 weeks. Compressive and tensile mechanical properties, GAG and collagen content, histology for GAG and collagen, and immunohistochemistry for collagen types I and II were assessed. When a culture control identified in phase I was used in phase II, HP application from 10 to 14 days resulted in a significant 1.4-fold increase in aggregate modulus, accompanied by an increase in GAG content, while HP application at all timepoints enhanced tensile properties and collagen content. In phase III, HP had an immediate effect on GAG content, collagen content, and compressive stiffness, while there was a delayed increase in tensile stiffness. The enhanced tensile stiffness was still present at 8 weeks. For the first time, this study examined the immediate and long-term effects of HP on biomechanical properties, and demonstrated that HP has an optimal application time in construct development. These findings are exciting as HP stimulation allowed for the formation of robust tissue-engineered cartilage; for example, 10 MPa static HP resulted in an aggregate modulus of 273 ± 123 kPa, a Young’s modulus of 1.6 ±0.4 MPa, a GAG/wet weight of 6.1 ±1.4%, and a collagen/wet weight of 10.6 ±2.4% at 4 weeks.
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影响因子：
3.3
作者：
Furukawa, KS;Suenaga, H;Tateishi, T
通讯作者：
Tateishi, T
DOI：
10.1115/1.3138202
发表时间：
1980-01-01
影响因子：
1.7
作者：
MOW, VC;KUEI, SC;ARMSTRONG, CG
通讯作者：
ARMSTRONG, CG
影响因子：
2.8
作者：
ATHANASIOU, KA;AGARWAL, A;DZIDA, FJ
通讯作者：
DZIDA, FJ
DOI：
10.2519/jospt.1998.28.4.192
发表时间：
1998-10-01
影响因子：
6.1
作者：
Buckwalter, JA
通讯作者：
Buckwalter, JA
影响因子：
5.5
作者：
Mizuno, S
通讯作者：
Mizuno, S