Molecular characterization and immune functional analysis of IRF2 in common carp (Cyprinus carpio L.): different regulatory role in the IFN and NF-κB signalling pathway.
Molecular characterization and immune functional analysis of IRF2 in common carp (Cyprinus carpio L.): different regulatory role in the IFN and NF-κB signalling pathway.
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DOI：
10.1186/s12917-021-03012-7
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发表时间：
2021-09-09
影响因子：
2.6
通讯作者：
Yang G
中科院分区：
文献类型：
--
作者：
Li H;Chen X;Zhu Y;Liu R;Zheng L;Shan S;Zhang F;An L;Yang G
干扰素调节因子2 （Interferon regulatory factor 2, IRF2）是一种重要的转录因子，可以调节IFN应答，在硬骨鱼的抗病毒先天免疫中发挥作用。本研究在鲤鱼（Cyprinus carpio L.）中鉴定了IRF2的全长cDNA序列（CcIRF2），该序列编码一个含有保守dna结合域（DBD）和irf相关域（IAD）的蛋白。系统发育分析表明，CcIRF2与Ctenopharyngodon idella的IRF2亲缘关系最为密切。在所有被检查的组织中都检测到CcIRF2转录本，在鳃、脾脏和脑中表达较高。外周血白细胞（pbl）和头肾白细胞（hkl）中多肌苷、多胞酸（poly (I:C)）和嗜水气单胞菌刺激以及多聚（I:C）、脂多糖（LPS）、肽聚糖（PGN）和鞭毛蛋白诱导下，CcIRF2在免疫相关组织中的表达上调。此外，过表达CcIRF2可降低IFN和IFN刺激基因（ISGs）的表达，双荧光素酶报告基因实验显示，CcIRF2可增加NF-κB的活化。这些结果表明，CcIRF2参与抗病毒和抗菌免疫反应，并负向调控IFN反应，为了解鲤鱼IFN系统的调控提供了新的认识，有助于鲤鱼养殖中传染病的预防和控制。
Interferon regulatory factor 2 (IRF2) is an important transcription factor, which can regulate the IFN response and plays a role in antiviral innate immunity in teleost. In the present study, the full-length cDNA sequence of IRF2 (CcIRF2) was characterized in common carp (Cyprinus carpio L.), which encoded a protein containing a conserved DNA-binding domain (DBD) and an IRF-associated domain (IAD). Phylogenetic analysis showed that CcIRF2 was most closely related with IRF2 of Ctenopharyngodon idella. CcIRF2 transcripts were detectable in all examined tissues, with higher expression in the gills, spleen and brain. CcIRF2 expression was upregulated in immune-related tissues of common carp upon polyinosinic:polycytidylic acid (poly (I:C)) and Aeromonas hydrophila stimulation and induced by poly (I:C), lipopolysaccharide (LPS), peptidoglycan (PGN) and flagellin in the peripheral blood leucocytes (PBLs) and head kidney leukocytes (HKLs). In addition, overexpression of CcIRF2 decreased the expression of IFN and IFN-stimulated genes (ISGs), and a dual-luciferase reporter assay revealed that CcIRF2 could increase the activation of NF-κB. These results indicate that CcIRF2 participates in antiviral and antibacterial immune response and negatively regulates the IFN response, which provide a new insight into the regulation of IFN system in common carp, and are helpful for the prevention and control of infectious diseases in carp farming.
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I型Interferon自分泌 - 核酸环与树突状细胞的Toll样受体诱导的白介素-12p70分泌有关。
影响因子：
15.3
作者：
Gautier, G;Humbert, M;Deauvieau, F;Scuiller, M;Hiscott, J;Bates, EEM;Trinchieri, G;Caux, C;Garrone, P
通讯作者：
Garrone, P
影响因子：
2.5
作者：
Rodkhum, C;Hirono, I;Aoki, T
通讯作者：
Aoki, T
影响因子：
2.9
作者：
Collet, B;Hovens, GCJ;Secombes, CJ
通讯作者：
Secombes, CJ
DOI：
10.1073/pnas.87.10.3743
发表时间：
1990-05-01
影响因子：
11.1
作者：
DRIGGERS, PH;ENNIST, DL;OZATO, K
通讯作者：
OZATO, K
影响因子：
3
作者：
Huang B;Qi ZT;Xu Z;Nie P
通讯作者：
Nie P