The lethal cargo of Myxococcus xanthus outer membrane vesicles.
The lethal cargo of Myxococcus xanthus outer membrane vesicles.
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DOI：
10.3389/fmicb.2014.00474
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发表时间：
2014
影响因子：
5.2
通讯作者：
Auer M
中科院分区：
文献类型：
--
作者：
Berleman JE;Allen S;Danielewicz MA;Remis JP;Gorur A;Cunha J;Hadi MZ;Zusman DR;Northen TR;Witkowska HE;Auer M
黄色粘球菌是一种细菌微型捕食者，以狼群般的方式捕食其他微生物而闻名。外膜囊泡（OMV）是由M.在细胞外环境中具有高度组织化的结构，有时在连接生物膜内相邻细胞的链中出现。OMV可能是介导狼群活动的一种载体，通过传递水解酶和抗生素来杀死猎物微生物。在此，OMV的蛋白质和小分子货物以及M. xanthus进行了表征和比较。我们的分析表明，一些蛋白质是OMV特异性或OMV富集，包括几个推定的水解功能。OMV的次级代谢产物谱鉴定了16种分子，其中许多与抗生素活性相关。鉴定了几种水解酶同源物，包括由MXAN_3564（mepA）编码的蛋白质，M36蛋白酶同源物。mepA的遗传破坏导致胞外蛋白酶活性的显著降低，表明MepA是M. xanthus
Myxococcus xanthus is a bacterial micro-predator known for hunting other microbes in a wolf pack-like manner. Outer membrane vesicles (OMVs) are produced in large quantities by M. xanthus and have a highly organized structure in the extracellular milieu, sometimes occurring in chains that link neighboring cells within a biofilm. OMVs may be a vehicle for mediating wolf pack activity by delivering hydrolytic enzymes and antibiotics aimed at killing prey microbes. Here, both the protein and small molecule cargo of the OMV and membrane fractions of M. xanthus were characterized and compared. Our analysis indicates a number of proteins that are OMV-specific or OMV-enriched, including several with putative hydrolytic function. Secondary metabolite profiling of OMVs identifies 16 molecules, many associated with antibiotic activities. Several hydrolytic enzyme homologs were identified, including the protein encoded by MXAN_3564 (mepA), an M36 protease homolog. Genetic disruption of mepA leads to a significant reduction in extracellular protease activity suggesting MepA is part of the long-predicted (yet to date undetermined) extracellular protease suite of M. xanthus.
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影响因子：
7.5
作者：
Cornejo, Elias;Abreu, Nicole;Komeili, Arash
通讯作者：
Komeili, Arash
影响因子：
11.3
作者：
Berleman JE;Kirby JR
通讯作者：
Kirby JR
影响因子：
7
作者：
Ishihama, Y;Oda, Y;Mann, M
通讯作者：
Mann, M
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作者：
Kim, Jiyoung;Choi, Jung Nam;Lee, Choong Hwan
通讯作者：
Lee, Choong Hwan
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作者：
Lilly, Walt W.;Stajich, Jason E.;Gathman, Allen C.
通讯作者：
Gathman, Allen C.