Two Cassava Basic Leucine Zipper (bZIP) Transcription Factors (MebZIP3 and MebZIP5) Confer Disease Resistance against Cassava Bacterial Blight.
Two Cassava Basic Leucine Zipper (bZIP) Transcription Factors (MebZIP3 and MebZIP5) Confer Disease Resistance against Cassava Bacterial Blight.
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DOI：
10.3389/fpls.2017.02110
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发表时间：
2017
影响因子：
5.6
通讯作者：
Shi H
中科院分区：
文献类型：
--
作者：
Li X;Fan S;Hu W;Liu G;Wei Y;He C;Shi H
碱性域亮氨酸拉链（bZIP）转录因子是一类保守基因家族，在植物发育和逆境响应中发挥着重要作用。尽管已在木薯全基因组范围内鉴定出 77 个 MebZIP，但它们的体内作用仍然未知。在本研究中，我们分析了两种 MebZIP（MebZIP3 和 MebZIP5）响应病原体感染的表达模式和功能。基因表达分析表明，MebZIP3 和 MebZIP5 普遍受到 Xanthomonas axonopodis pv. flg22 的调控。木薯 (Xam)、水杨酸 (SA) 和过氧化氢 (H2O2)。亚细胞定位分析表明MebZIP3和MebZIP5特异性定位于细胞核。通过在烟草中过度表达，我们发现 MebZIP3 和 MebZIP5 提高了木薯白叶枯病的抗病性，并具有更多的胼胝质沉积。相反，通过病毒诱导基因沉默 (VIGS) 沉默的 MebZIP3 和 MebZIP5 植物表现出疾病敏感表型、防御相关基因转录水平较低和胼胝质沉积较少。综上所述，本研究强调了 MebZIP3 和 MebZIP5 在木薯白叶枯病抗性中的积极作用，以进一步用于木薯抗病性的遗传改良。
Basic domain-leucine zipper (bZIP) transcription factor, one type of conserved gene family, plays an important role in plant development and stress responses. Although 77 MebZIPs have been genome-wide identified in cassava, their in vivo roles remain unknown. In this study, we analyzed the expression pattern and the function of two MebZIPs (MebZIP3 and MebZIP5) in response to pathogen infection. Gene expression analysis indicated that MebZIP3 and MebZIP5 were commonly regulated by flg22, Xanthomonas axonopodis pv. manihotis (Xam), salicylic acid (SA), and hydrogen peroxide (H2O2). Subcellular localization analysis showed that MebZIP3 and MebZIP5 are specifically located in cell nucleus. Through overexpression in tobacco, we found that MebZIP3 and MebZIP5 conferred improved disease resistance against cassava bacterial blight, with more callose depositions. On the contrary, MebZIP3- and MebZIP5-silenced plants by virus-induced gene silencing (VIGS) showed disease sensitive phenotype, lower transcript levels of defense-related genes and less callose depositions. Taken together, this study highlights the positive role of MebZIP3 and MebZIP5 in disease resistance against cassava bacterial blight for further utilization in genetic improvement of cassava disease resistance.
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