Protocatechuic acid inhibits the growth of ovarian cancer cells by inducing apoptosis and autophagy
Protocatechuic acid inhibits the growth of ovarian cancer cells by inducing apoptosis and autophagy
复制标题
原儿茶酸通过诱导细胞凋亡和自噬抑制卵巢癌细胞的生长
DOI：
10.1002/ptr.6163
复制
发表时间：
2018-07
影响因子：
7.2
通讯作者：
Jianqing Yu
中科院分区：
文献类型：
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作者：
Zhoufan Xie;Ziyan Guo;Yun Wang;Jiachuan Lei;Jianqing Yu
原儿茶酸(PCA)存在于多种水果和蔬菜中，具有多种生物活性。在这里，我们提供了证据，表明它可以开发为一种潜在的抗人卵巢癌的化疗药物。我们发现PCA处理显著降低了OVCAR-3、SKOV-3和A2780细胞的细胞存活率和集落形成。选择OVCAR-3细胞作为研究分子机制的实验模型系统。PCA诱导OVCAR-3细胞周期停滞于G2/M期，多聚腺苷二磷酸核糖聚合酶(PARP)和半胱氨酸天冬氨酸氨基转移酶-3(caspase-3)激活，Bax表达上调，Bcl一2表达下调。我们还观察到，PCA处理显著上调了自噬相关蛋白LC3-II，并诱导了GFP-LC3点的形成。此外，PCA和自噬抑制剂共同作用可减弱PCA对OVCAR-3细胞的细胞毒作用。此外，我们的结果显示，PCA可增加细胞内谷胱甘肽水平，降低细胞内活性氧，这可能与PCA对OVCAR-3细胞的抑制作用有关。我们的数据显示，PCA可以调节细胞的凋亡和自噬，提示PCA在卵巢癌的化学预防和化疗中具有潜在的潜力。
Protocatechuic acid (PCA), present in many fruits and vegetables, exhibited various biological activities. Here, we provided evidence that it could be developed as a potential chemotherapeutic agent against human ovarian cancer. We found that PCA treatment significantly reduced the cell viability and colony formation of OVCAR‐3, SKOV‐3, and A2780 cells. OVCAR‐3 cells were selected as a test model system for investigating molecular mechanism. PCA treatment induced cell cycle arrest in G2/M phase, the activation of poly (ADP‐ribose) polymerase (PARP) and caspase‐3, the upregulation of Bax and downregulation of Bcl‐2 in OVCAR‐3 cells. We also observed that PCA treatment significantly caused upregulation of autophagy‐related protein LC3‐II and induced GFP‐LC3 puncta formation. Furthermore, cotreatment with PCA and autophagy inhibitor attenuated the cytotoxicity induced by PCA in OVCAR‐3 cells. Moreover, our results showed that PCA increased the intracellular levels of glutathione and decreased intracellular reactive oxygen species that might be related to the inhibition effect of PCA on OVCAR‐3 cells. Our data revealed that PCA could modulate apoptosis and autophagy, suggesting the potential of PCA for chemoprevention and chemotherapy of ovarian cancer.
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DOI：
10.1155/2015/865986
发表时间：
2015
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Xuan M;Okazaki M;Iwata N;Asano S;Kamiuchi S;Matsuzaki H;Sakamoto T;Miyano Y;Iizuka H;Hibino Y
通讯作者：
Hibino Y
DOI：
10.1016/j.fct.2015.12.005
发表时间：
2016-02
期刊：
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
影响因子：
--
作者：
Shenli Wang;R. Mateos;L. Goya;Miryam Amigo-Benavent;B. Sarriá;L. Bravo
通讯作者：
Shenli Wang;R. Mateos;L. Goya;Miryam Amigo-Benavent;B. Sarriá;L. Bravo
影响因子：
3.3
作者：
Guqi Wang;Y. Gong;F. Burczynski;B. Hasinoff
通讯作者：
Guqi Wang;Y. Gong;F. Burczynski;B. Hasinoff
影响因子：
3
作者：
B. Zingarelli
通讯作者：
B. Zingarelli
影响因子：
6.1
作者：
Tian, S;Nakamura, K;Kayahara, H
通讯作者：
Kayahara, H