Testicular macrophages are recruited during a narrow fetal time window and promote organ-specific developmental functions.
Testicular macrophages are recruited during a narrow fetal time window and promote organ-specific developmental functions.
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DOI：
10.1038/s41467-023-37199-0
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发表时间：
2023-03-15
影响因子：
16.6
通讯作者：
DeFalco, Tony
中科院分区：
文献类型：
--
作者：
Gu, Xiaowei;Heinrich, Anna;Li, Shu-Yun;DeFalco, Tony
越来越多的证据表明，胎儿来源的组织驻留巨噬细胞具有发育功能。有人提出巨噬细胞促进睾丸功能，但涉及哪些巨噬细胞群体尚不清楚。先前的研究表明，巨噬细胞在胎儿睾丸形态发生中起关键作用，并描述了两个成年睾丸巨噬细胞群体，间质和管周。关于睾丸巨噬细胞的造血起源以及不同的巨噬细胞群体是否促进特定的睾丸功能一直存在争议。在这里，我们的小鼠造血谱系追踪研究表明，卵黄囊来源的巨噬细胞包括最早的睾丸巨噬细胞，而胎儿造血干细胞（HSC）产生单核细胞，在一个狭窄的时间窗内以支持细胞依赖的方式定殖性腺，并分化成成年睾丸巨噬细胞。最后，我们发现，卵黄囊源性与HSC源性巨噬细胞在睾丸形态发生过程中有不同的功能，而间质巨噬细胞特异性地促进成年Leydig细胞类固醇生成。我们的研究结果提供了深入了解睾丸巨噬细胞的起源和它们的组织特异性作用。睾丸巨噬细胞如何发育并影响组织功能尚未完全了解。在这里，作者使用小鼠谱系追踪方法来记录造血来源，早期睾丸巨噬细胞的发育和募集，胎儿睾丸分化的支持，以及与Leydig细胞的相互作用和促进类固醇生成。
A growing body of evidence demonstrates that fetal-derived tissue-resident macrophages have developmental functions. It has been proposed that macrophages promote testicular functions, but which macrophage populations are involved is unclear. Previous studies showed that macrophages play critical roles in fetal testis morphogenesis and described two adult testicular macrophage populations, interstitial and peritubular. There has been debate regarding the hematopoietic origins of testicular macrophages and whether distinct macrophage populations promote specific testicular functions. Here our hematopoietic lineage-tracing studies in mice show that yolk-sac-derived macrophages comprise the earliest testicular macrophages, while fetal hematopoietic stem cells (HSCs) generate monocytes that colonize the gonad during a narrow time window in a Sertoli-cell-dependent manner and differentiate into adult testicular macrophages. Finally, we show that yolk-sac-derived versus HSC-derived macrophages have distinct functions during testis morphogenesis, while interstitial macrophages specifically promote adult Leydig cell steroidogenesis. Our findings provide insight into testicular macrophage origins and their tissue-specific roles. How testis resident macrophages develop and influence tissue function is not fully understood. Here the authors use mouse lineage tracing methods to document the haematopoietic source, development and recruitment of early testicular macrophages, support of foetal testis differentiation, and interaction with, and promotion of steroidogenesis in, Leydig cells.
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影响因子：
16.2
作者：
Dhillon, H;Zigman, JM;Lowelll, BB
通讯作者：
Lowelll, BB
DOI：
10.1007/978-1-60761-869-0_29
发表时间：
2011-01-01
期刊：
SUPPRESSION AND REGULATION OF IMMUNE RESPONSES: METHODS AND PROTOCOLS
影响因子：
--
作者：
Fijak, Monika;Bhushan, Sudhanshu;Meinhardt, Andreas
通讯作者：
Meinhardt, Andreas
影响因子：
8.8
作者：
DeFalco T;Potter SJ;Williams AV;Waller B;Kan MJ;Capel B
通讯作者：
Capel B
影响因子：
4.4
作者：
Grabert, Kathleen;Sehgal, Anuj;Hume, David A.
通讯作者：
Hume, David A.
DOI：
10.1038/mt.2014.112
发表时间：
2014-09
期刊：
Molecular therapy : the journal of the American Society of Gene Therapy
影响因子：
--
作者：
Gow DJ;Sauter KA;Pridans C;Moffat L;Sehgal A;Stutchfield BM;Raza S;Beard PM;Tsai YT;Bainbridge G;Boner PL;Fici G;Garcia-Tapia D;Martin RA;Oliphant T;Shelly JA;Tiwari R;Wilson TL;Smith LB;Mabbott NA;Hume DA
通讯作者：
Hume DA