Preterm Infant-Associated Clostridium tertium, Clostridium cadaveris, and Clostridium paraputrificum Strains: Genomic and Evolutionary Insights.
Preterm Infant-Associated Clostridium tertium, Clostridium cadaveris, and Clostridium paraputrificum Strains: Genomic and Evolutionary Insights.
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DOI：
10.1093/gbe/evx210
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发表时间：
2017-10-01
影响因子：
3.3
通讯作者：
Hall LJ
中科院分区：
文献类型：
--
作者：
Kiu R;Caim S;Alcon-Giner C;Belteki G;Clarke P;Pickard D;Dougan G;Hall LJ
梭菌种类（特别是艰难梭菌、肉毒梭菌、破伤风梭菌和产气荚膜梭菌）与一系列人类和动物疾病有关。包括tertium梭状芽孢杆菌（Clostridium cadaveris）、尸体梭状芽孢杆菌（Clostridium cadaveris）和副干酪梭状芽孢杆菌（Clostridium paraputrificum）在内的其他几种梭状芽孢杆菌也与散发的人类感染有关，然而，这些研究非常有限，或者在某些情况下，没有公开的基因组信息。因此，我们从新生儿重症监护病房的早产儿中分离出1株C. tertium菌株、1株C. cadaveris菌株和3株C. paraputrificum菌株，并使用Illumina HiSeq进行全基因组测序（WGS）。在本报告中，我们宣布基因组草案：C. tertium LH009、C. cadaveris LH052、C. paraputrificum LH025、C. paraputrificum LH058和C. paraputrificum LH141。我们利用16S rRNA基因序列（来自PCR和PCR）和基于wgs的方法确认了物种的身份和系统发育。使用平均核苷酸同一性（ANI）来区分其最近亲缘的基因组，以进一步确定物种形成边界。我们还分析了毒力相关因子和抗菌素耐药基因的基因组，并检测了四环素和甲氧西林耐药性的存在，以及潜在的有害酶，包括多种磷脂酶和毒素。开放数据库中基因组数据的可用性，以及我们对这些致病性梭状芽孢杆菌物种的基因组内容和毒力特征的初步见解，应使科学界能够进一步研究这些细菌的致病机制，以加强临床诊断和治疗。
Clostridium species (particularly Clostridium difficile, Clostridium botulinum, Clostridium tetani and Clostridium perfringens) are associated with a range of human and animal diseases. Several other species including Clostridium tertium, Clostridium cadaveris, and Clostridium paraputrificum have also been linked with sporadic human infections, however there is very limited, or in some cases, no genomic information publicly available. Thus, we isolated one C. tertium strain, one C. cadaveris strain and three C. paraputrificum strains from preterm infants residing within neonatal intensive care units and performed Whole Genome Sequencing (WGS) using Illumina HiSeq. In this report, we announce the open availability of the draft genomes: C. tertium LH009, C. cadaveris LH052, C. paraputrificum LH025, C. paraputrificum LH058, and C. paraputrificum LH141. These genomes were checked for contamination in silico to ensure purity, and we confirmed species identity and phylogeny using both 16S rRNA gene sequences (from PCR and in silico) and WGS-based approaches. Average Nucleotide Identity (ANI) was used to differentiate genomes from their closest relatives to further confirm speciation boundaries. We also analysed the genomes for virulence-related factors and antimicrobial resistance genes, and detected presence of tetracycline and methicillin resistance, and potentially harmful enzymes, including multiple phospholipases and toxins. The availability of genomic data in open databases, in tandem with our initial insights into the genomic content and virulence traits of these pathogenic Clostridium species, should enable the scientific community to further investigate the disease-causing mechanisms of these bacteria with a view to enhancing clinical diagnosis and treatment.
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影响因子：
10.7
作者：
Huerta-Cepas J;Forslund K;Coelho LP;Szklarczyk D;Jensen LJ;von Mering C;Bork P
通讯作者：
Bork P
影响因子：
14.9
作者：
Geer LY;Marchler-Bauer A;Geer RC;Han L;He J;He S;Liu C;Shi W;Bryant SH
通讯作者：
Bryant SH
影响因子：
4.9
作者：
Golparian, Daniel;Shafer, William M.;Unemo, Magnus
通讯作者：
Unemo, Magnus
影响因子：
3.2
作者：
Evers, S;Courvalin, P
通讯作者：
Courvalin, P
影响因子：
5.2
作者：
Horiyama, Tsukasa;Nikaido, Eiji;Nishino, Kunihiko
通讯作者：
Nishino, Kunihiko