Role of Endoplasmic Reticulum Stress in Otitis Media
Role of Endoplasmic Reticulum Stress in Otitis Media
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内质网应激在中耳炎中的作用
DOI：
10.3389/fgene.2020.00495
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发表时间：
2020-05
影响因子：
3.7
通讯作者：
Zheng Qingyin
中科院分区：
文献类型：
--
作者：
Zhao Hongchun;Wang Yanfei;Li Bo;Zheng Tihua;Liu Xiuzhen;Hu Bo Hua;Che Juan;Zhao Tong;Chen Jun;Hatzoglou Maria;Zhang Xiaolin;Fan Zhaomin;Zheng Qingyin
Endoplasmic reticulum (ER) stress occurs in many inflammatory responses. Here, we investigated the role of ER stress and its associated apoptosis in otitis media (OM) to elucidate the mechanisms of OM and the signaling crosstalk between ER stress and other cell damage pathways, including inflammatory cytokines and apoptosis. We examined the expression of inflammatory cytokine- and ER stress-related genes by qRT-PCR, Western blotting, and immunohistochemistry (IHC) in the middle ear of C57BL/6J mice after challenge with peptidoglycan polysaccharide (PGPS), an agent inducing OM. We also evaluated the effect of the suppression of ER stress with tauroursodeoxycholic acid (TUDCA), an ER stress inhibitor. The study revealed the upregulation of ER stress- and apoptosis-related gene expression after the PGPS treatment, specifically ATF6, CHOP, BIP, caspase-12, and caspase-3. TUDCA treatment of PGPS-treated mice decreased OM; reduced the expression of CHOP, BIP, and caspase 3; and significantly decreased the proinflammatory gene expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). These results suggest that PGPS triggers ER stress and downstream proinflammatory gene expression in OM and that inhibition of ER stress alleviates OM. We propose that ER stress plays a critical role in inflammation and cell death, leading to the development of OM and points to ER stress inhibition as a potential therapeutic approach for the prevention of OM.
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影响因子：
158.5
作者：
Hendley, JO
通讯作者：
Hendley, JO
影响因子：
3.3
作者：
Han F;Yu H;Zheng T;Ma X;Zhao X;Li P;Le L;Su Y;Zheng QY
通讯作者：
Zheng QY
影响因子：
4.4
作者：
A. Yoshimura;E. Lien;R. Ingalls;E. Tuomanen;R. Dziarski;D. Golenbock
通讯作者：
A. Yoshimura;E. Lien;R. Ingalls;E. Tuomanen;R. Dziarski;D. Golenbock
影响因子：
16
作者：
Guan BJ;van Hoef V;Jobava R;Elroy-Stein O;Valasek LS;Cargnello M;Gao XH;Krokowski D;Merrick WC;Kimball SR;Komar AA;Koromilas AE;Wynshaw-Boris A;Topisirovic I;Larsson O;Hatzoglou M
通讯作者：
Hatzoglou M
影响因子：
1.2
作者：
Pia Nitsche, Maria;Carreno, Monica
通讯作者：
Carreno, Monica