Characterization of bovine induced pluripotent stem cells by lentiviral transduction of reprogramming factor fusion proteins.
Characterization of bovine induced pluripotent stem cells by lentiviral transduction of reprogramming factor fusion proteins.
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通过慢病毒转导重编程因子融合蛋白来表征牛诱导多能干细胞
DOI：
10.7150/ijbs.3723
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发表时间：
2012
影响因子：
9.2
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Cao H;Yang P;Pu Y;Sun X;Yin H;Zhang Y;Zhang Y;Li Y;Liu Y;Fang F;Zhang Z;Tao Y;Zhang X
家畜多能干细胞在转基因育种中具有潜在的应用价值。在这里，我们描述了诱导多能干细胞（iPS）来自牛胎儿成纤维细胞的Oct 4，Sox 2，Klf 4和c-Myc定义的因子融合蛋白的慢病毒转导。牛iPS细胞具有典型的集落形态，核型正常，碱性磷酸酶（AP）染色阳性，表达Oct 4、Nanog和SSEA 1。与成纤维细胞相比，在牛iPS细胞中Oct 4和Nanog的启动子区域中的CpG高度未甲基化。细胞能够分化成所有三个胚层的细胞类型在体外和体内。此外，这些细胞在确定的培养条件下被诱导为雌性生殖细胞，并表达早期和晚期雌性生殖细胞特异性基因Vasa、Dazl、Gdf 9、Nobox、Zp 2和Zp 3。我们的数据表明，牛iPS细胞与慢病毒介导的确定因子融合蛋白从牛胎儿成纤维细胞中产生，并在牛转基因育种和基因修饰动物中具有潜在的应用。
Pluripotent stem cells from domesticated animals have potential applications in transgenic breeding. Here, we describe induced pluripotent stem (iPS) cells derived from bovine fetal fibroblasts by lentiviral transduction of Oct4, Sox2, Klf4 and c-Myc defined-factor fusion proteins. Bovine iPS cells showed typical colony morphology, normal karyotypes, stained positively for alkaline phosphatase (AP) and expressed Oct4, Nanog and SSEA1. The CpG in the promoter regions of Oct4 and Nanog were highly unmethylated in bovine iPS cells compared to the fibroblasts. The cells were able to differentiate into cell types of all three germ layers in vitro and in vivo. In addition, these cells were induced into female germ cells under defined culture conditions and expressed early and late female germ cell-specific genes Vasa, Dazl, Gdf9, Nobox, Zp2, and Zp3. Our data suggest that bovine iPS cells were generated from bovine fetal fibroblasts with defined-factor fusion proteins mediated by lentivirus and have potential applications in bovine transgenic breeding and gene-modified animals.
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评估合适的参考基因用于牛肌肉组织中的基因表达研究。
影响因子：
--
作者：
Pérez R;Tupac-Yupanqui I;Dunner S
通讯作者：
Dunner S
DOI：
10.1089/clo.2007.0031
发表时间：
2007-12-01
期刊：
CLONING AND STEM CELLS
影响因子：
--
作者：
Kerkis, Alexandre;Fonseca, Simone A. S.;Kerkis, Irina
通讯作者：
Kerkis, Irina
影响因子：
4.8
作者：
Esteban, Miguel A.;Xu, Jianyong;Pei, Duanqing
通讯作者：
Pei, Duanqing
影响因子：
64.5
作者：
Takahashi, Kazutoshi;Yamanaka, Shinya
通讯作者：
Yamanaka, Shinya
影响因子：
56.9
作者：
Yu, Junying;Vodyanik, Maxim A.;Thomson, James A.
通讯作者：
Thomson, James A.