IMP-1 encoded by a novel Tn402-like class 1 integron in clinical Achromobacter xylosoxidans, China.

IMP-1 encoded by a novel Tn402-like class 1 integron in clinical Achromobacter xylosoxidans, China.
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DOI:
10.1038/srep07212
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发表时间:
2014-11-27
期刊:
影响因子:
4.6
通讯作者:
Liu C
Liu C
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Chen Z;Fang H;Wang L;Sun F;Wang Y;Yin Z;Yang H;Yang W;Wang J;Xia P;Zhou D;Liu C

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木糖氧化无色杆菌菌株A22732分离自中国的肺炎患者,并产生碳青霉烯酶OXA-114 e和IMP-1,其分别由染色体和质粒编码,并赋予对包括碳青霉烯类在内的多种β-内酰胺类抗生素的耐药性。blaIMP-1基因与aacA 7和orfE一起被接合IncP-1质粒中的新型Tn 402样1类整合子捕获。除了固有的整合子启动子PcW外,还有blaIMP-1基因盒特异性启动子。这是首次在临床分离的细菌中发现编码碳青霉烯酶的IncP-1 β质粒。
Achromobacter xylosoxidans strain A22732 is isolated from a pneumonia patient in China and produces carbapenemases OXA-114e and IMP-1, which are encoded by chromosome and plasmid, respectively, and confer resistance to multiple ß-lactam antibiotics including carbapenems. The blaIMP-1 gene together with aacA7 and orfE is captured by a novel Tn402-like class 1 integron in a conjugative IncP-1ß plasmid. In addition to the intrinsic integron promoter PcW, there is still a blaIMP-1 gene cassette-specific promoter. This is the first report of carbapenemase-encoding IncP-1ß plasmid in clinical bacterial isolate.
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