Gene expression patterns that support novel developmental stress buffering in embryos of the annual killifish Austrofundulus limnaeus.
Gene expression patterns that support novel developmental stress buffering in embryos of the annual killifish Austrofundulus limnaeus.
复制标题
DOI：
10.1186/2041-9139-6-2
复制
发表时间：
2015
期刊：
影响因子：
4.1
通讯作者：
Podrabsky JE
中科院分区：
文献类型：
--
作者：
Wagner JT;Podrabsky JE
在脊椎动物中，轴形成和原肠胚形成所涉及的细胞信号传导机制和形态发生运动是非常保守的。在几乎所有描述的鱼，原肠胚和胚胎轴的初始图案发生同时与外包。然而，每年的鳉鱼可能是这一常态的例外。一年生的鳉鱼栖息在南美洲和非洲的临时池塘，永久种群通过生产耐压力的卵而持续存在。一年生鳉鱼的早期发育在脊椎动物中是独特的，因为它们的胚胎卵裂球在胚胎发育过程中随机分散在卵黄中，并在几天后重新聚集形成胚胎。此外，一年生鳉鱼能够在一至三个阶段阻止胚胎发育，称为滞育I，II和III。鲜为人知的是，如何高度保守的发展与早期脊椎动物的发展信号机制可能已经改变，以促进每年的鳉鱼表型。其中一个最好的特点和保守的转录因子，oct 4（Pou 5 f1），可能有一个在维持多能性的作用。相反，BMP-拮抗剂，如脊索蛋白，头蛋白，卵泡抑素，先前已被证明建立轴形成过程中的背腹不对称。来自SOXB 1组的转录因子，如sox 2和sox 3，可能会诱导神经特化。在这里，我们确定的时间表达这些发育因子在胚胎发育过程中的年度澳底鱼limnaeus使用定量PCR和比较这些模式与其他脊椎动物。对oct 4、sox 2、sox 3、chordin、noggin-1、noggin-2和follistatin的部分转录序列进行克隆、测序，并在A.利姆奈我们发现oct 4，sox 3，chordin和noggin-1的转录本可能是母系遗传的。sox 2，卵泡抑素和noggin-2的转录表达最高的阶段后，可见的胚胎轴。我们的数据表明，胚胎细胞获得其胚层身份后，胚胎卵裂球重新聚集在A。利姆奈这种细胞分化和轴形成的过程可能涉及与其他脊椎动物相似的保守信号传导机制。我们建议，未分化状态在卵裂球分散过程中延长，从而在胚胎细胞之间建立胚胎不对称性和位置认同之前作为发育压力缓冲器发挥作用。本文的在线版本（doi：10.1186/2041-9139-6-2）包含补充材料，可供授权用户使用。
The cellular signaling mechanisms and morphogenic movements involved in axis formation and gastrulation are well conserved between vertebrates. In nearly all described fish, gastrulation and the initial patterning of the embryonic axis occur concurrently with epiboly. However, annual killifish may be an exception to this norm. Annual killifish inhabit ephemeral ponds in South America and Africa and permanent populations persist by the production of stress-tolerant eggs. Early development of annual killifish is unique among vertebrates because their embryonic blastomeres disperse randomly across the yolk during epiboly and reaggregate several days later to form the embryo proper. In addition, annual killifish are able to arrest embryonic development in one to three stages, known as diapause I, II, and III. Little is known about how the highly conserved developmental signaling mechanisms associated with early vertebrate development may have shifted in order to promote the annual killifish phenotype. One of the most well-characterized and conserved transcription factors, oct4 (Pou5f1), may have a role in maintaining pluripotency. In contrast, BMP-antagonists such as chordin, noggin, and follistatin, have been previously shown to establish dorsal-ventral asymmetry during axis formation. Transcription factors from the SOXB1 group, such as sox2 and sox3, likely work to induce neural specification. Here, we determine the temporal expression of these developmental factors during embryonic development in the annual killifish Austrofundulus limnaeus using quantitative PCR and compare these patterns to other vertebrates. Partial transcript sequences to oct4, sox2, sox3, chordin, noggin-1, noggin-2, and follistatin were cloned, sequenced, and identified in A. limnaeus. We found oct4, sox3, chordin, and noggin-1 transcripts to likely be maternally inherited. Expression of sox2, follistatin, and noggin-2 transcripts were highest in stages following a visible embryonic axis. Our data suggest that embryonic cells acquire their germ layer identity following embryonic blastomere reaggregation in A. limnaeus. This process of cellular differentiation and axis formation may involve similar conserved signaling mechanisms to other vertebrates. We propose that the undifferentiated state is prolonged during blastomere dispersal, thus functioning as a developmental stress buffer prior to the establishment of embryonic asymmetry and positional identity among the embryonic cells. The online version of this article (doi:10.1186/2041-9139-6-2) contains supplementary material, which is available to authorized users.
登录
查看更多内容
DOI：
10.1073/pnas.95.16.9337
发表时间：
1998-08-04
影响因子：
11.1
作者：
Iemura, S;Yamamoto, TS;Ueno, N
通讯作者：
Ueno, N
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
影响因子：
2.7
作者：
Dal-Pra, Sophie;Fuerthauer, Maximilian;Thisse, Christine
通讯作者：
Thisse, Christine
影响因子：
2.7
作者：
Dee, Chris T.;Hirst, Caroline S.;Scotting, Paul J.
通讯作者：
Scotting, Paul J.
日本牙鲆中 Sox2 同源物的鉴定和表征
影响因子：
3.5
作者：
Gao, Jinning;Wang, Zhongkai;Zhang, Quanqi
通讯作者：
Zhang, Quanqi