Identifying the core genome of the nucleus-forming bacteriophage family and characterization of Erwinia phage RAY.
Identifying the core genome of the nucleus-forming bacteriophage family and characterization of Erwinia phage RAY.
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DOI：
10.1016/j.celrep.2023.112432
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发表时间：
2023-05-30
期刊：
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
我们最近发现，一些噬菌体建立了一个核样复制区室（噬菌体核），但定义基于核的噬菌体复制的核心基因及其系统发育分布仍有待确定。在这里，我们发现，编码主要噬菌体核蛋白嵌合蛋白的peptide共享72个保守的基因编码的7个基因块内。其中，21个核心基因是核形成噬菌体所特有的，除了一个基因之外，所有这些基因都编码功能未知的蛋白质。我们认为这些噬菌体组成了一个新的病毒家族，我们称之为Chimalliviridae。欧文氏菌噬菌体vB_EamM_RAY的荧光显微镜和冷冻电子断层扫描研究证实，许多基于核的复制的关键步骤是保守的不同嵌合病毒，并揭示了这种复制机制的变化。这项工作扩展了我们对噬菌体核和噬菌体纺锤体多样性和功能的理解，为确定基于核的噬菌体复制的关键机制提供了路线图。一些噬菌体在其感染周期中显示出核样结构（噬菌体核）。这些病毒分布广泛，感染许多宿主。Prichard等人发现，在系统发育树中，这些染色体聚在一起，表明所有已知的成核染色体都有共同的祖先。
We recently discovered that some bacteriophages establish a nucleus-like replication compartment (phage nucleus), but the core genes that define nucleus-based phage replication and their phylogenetic distribution were still to be determined. Here, we show that phages encoding the major phage nucleus protein chimallin share 72 conserved genes encoded within seven gene blocks. Of these, 21 core genes are unique to nucleus-forming phage, and all but one of these genes encode proteins of unknown function. We propose that these phages comprise a novel viral family we term Chimalliviridae. Fluorescence microscopy and cryoelectron tomography studies of Erwinia phage vB_EamM_RAY confirm that many of the key steps of nucleus-based replication are conserved among diverse chimalliviruses and reveal variations on this replication mechanism. This work expands our understanding of phage nucleus and PhuZ spindle diversity and function, providing a roadmap for identifying key mechanisms underlying nucleus-based phage replication. Some bacteriophages have been shown to make a nucleus-like structure (the phage nucleus) during their infection cycle. These phages are widespread and infect numerous hosts. Prichard et al. find that these phages cluster together in phylogenetic trees, suggesting a common ancestry of all known nucleus-forming phages.
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影响因子：
13.6
作者：
Chaikeeratisak, Vorrapon;Khanna, Kanika;Nguyen, Katrina T.;Egan, MacKennon E.;Enustun, Eray;Armbruster, Emily;Lee, Jina;Pogliano, Kit;Villa, Elizabeth;Pogliano, Joe
通讯作者：
Pogliano, Joe
影响因子：
8.8
作者：
Birkholz, Erica A.;Laughlin, Thomas G.;Armbruster, Emily;Suslov, Sergey;Lee, Jina;Wittmann, Johannes;Corbett, Kevin D.;Villa, Elizabeth;Pogliano, Joe
通讯作者：
Pogliano, Joe
影响因子：
6.5
作者：
Barylski, Jakub;Enault, Francois;Adriaenssens, Evelien M.
通讯作者：
Adriaenssens, Evelien M.
影响因子：
5.4
作者：
Ceyssens, Pieter-Jan;Minakhin, Leonid;Lavigne, Rob
通讯作者：
Lavigne, Rob
DOI：
10.1073/pnas.1010176107
发表时间：
2010-11-16
影响因子：
11.1
作者：
Aylett, Christopher H. S.;Wang, Qing;Loewe, Jan
通讯作者：
Loewe, Jan