A cytoskeletal vortex drives phage nucleus rotation during jumbo phage replication in E. coli.
A cytoskeletal vortex drives phage nucleus rotation during jumbo phage replication in E. coli.
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DOI：
10.1016/j.celrep.2022.111179
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发表时间：
2022-08-16
期刊：
影响因子：
8.8
通讯作者：
Pogliano, Joe
中科院分区：
文献类型：
--
作者：
Birkholz, Erica A.;Laughlin, Thomas G.;Armbruster, Emily;Suslov, Sergey;Lee, Jina;Wittmann, Johannes;Corbett, Kevin D.;Villa, Elizabeth;Pogliano, Joe
形成细胞核的巨型噬菌体建立了一个复杂的亚细胞组织，将噬菌体基因组包裹在一个称为噬菌体核的蛋白质外壳内。在感染假单胞菌的过程中，一些巨型噬菌体组装成微管蛋白样的双极纺锤体，将噬菌体核定位在中细胞并驱动其细胞内旋转。这有利于衣壳在其表面上的分布，用于基因组包装。在这里，我们表明，大肠杆菌巨型噬菌体Goslar组装一个噬菌体核周围的一个阵列，类似于一个漩涡，而不是一个双极纺锤体的czz丝。突变体BZZ蛋白的表达强烈地降低了Goslar噬菌体核旋转，表明BZZ细胞骨架涡旋对于旋转噬菌体核是必要的。虽然涡旋状细胞骨架阵列在真核生物中对于细胞质流动和细胞核对齐是重要的，但这项工作确定了原核细胞质内驱动细胞内旋转的丝状体连贯组装成涡旋状结构。噬菌体核是最近发现的，这种噬菌体复制途径的多样性和普遍性尚不清楚。Birkholz等鉴定了一个成核E.大肠杆菌噬菌体，并显示微管蛋白（Tubulin）（
Nucleus-forming jumbo phages establish an intricate subcellular organization, enclosing phage genomes within a proteinaceous shell called the phage nucleus. During infection in Pseudomonas, some jumbo phages assemble a bipolar spindle of tubulin-like PhuZ filaments that positions the phage nucleus at midcell and drives its intracellular rotation. This facilitates the distribution of capsids on its surface for genome packaging. Here we show that the Escherichia coli jumbo phage Goslar assembles a phage nucleus surrounded by an array of PhuZ filaments resembling a vortex instead of a bipolar spindle. Expression of a mutant PhuZ protein strongly reduces Goslar phage nucleus rotation, demonstrating that the PhuZ cytoskeletal vortex is necessary for rotating the phage nucleus. While vortex-like cytoskeletal arrays are important in eukaryotes for cytoplasmic streaming and nucleus alignment, this work identifies a coherent assembly of filaments into a vortex-like structure driving intracellular rotation within the prokaryotic cytoplasm. The phage nucleus was recently discovered, and the diversity and ubiquity of this phage replication pathway is unclear. Birkholz et al. identify a nucleus-forming E. coli phage and show that tubulin (PhuZ) filaments form a vortex-like structure driving intracellular rotation of the phage nucleus within the prokaryotic cytoplasm.
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影响因子：
13.6
作者：
Chaikeeratisak, Vorrapon;Khanna, Kanika;Nguyen, Katrina T.;Egan, MacKennon E.;Enustun, Eray;Armbruster, Emily;Lee, Jina;Pogliano, Kit;Villa, Elizabeth;Pogliano, Joe
通讯作者：
Pogliano, Joe
DOI：
10.3390/v13020149
发表时间：
2021-01-20
期刊：
Viruses
影响因子：
--
作者：
Krylov V;Bourkaltseva M;Pleteneva E;Shaburova O;Krylov S;Karaulov A;Zhavoronok S;Svitich O;Zverev V
通讯作者：
Zverev V
影响因子：
4
作者：
Levy JR;Holzbaur EL
通讯作者：
Holzbaur EL
影响因子：
10.5
作者：
Larsen, Rachel A.;Cusumano, Christina;Pogliano, Joe
通讯作者：
Pogliano, Joe
影响因子：
5.4
作者：
Ceyssens, Pieter-Jan;Minakhin, Leonid;Lavigne, Rob
通讯作者：
Lavigne, Rob