Tissue engineering of a composite trachea construct using autologous rabbit chondrocytes.
Tissue engineering of a composite trachea construct using autologous rabbit chondrocytes.
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使用自体软骨细胞的复合气管构建体的组织工程。
DOI：
10.1002/term.2523
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发表时间：
2018-03
影响因子：
3.3
通讯作者：
Meyer TK
中科院分区：
文献类型：
--
作者：
Dennis JE;Bernardi KG;Kean TJ;Liou NE;Meyer TK
大型气管节段性缺损的修复仍是一个悬而未决的问题。本研究的目的是将组织工程学原理应用于大型节段性气管替代物的制造。由自体细胞组成的工程化气管替代物(“新气管”)在新西兰大白兔模型上进行了测试。在兔颈部，用硅胶管包裹一层连续的皮肤上皮、颈阔肌和工程化软骨片，并使其成熟8-12周，形成新的气管。总共有28只兔被植入，并对新生气管进行了组织形态评估。在11例患者中，被认为足够坚固的新气管用于修复节段性气管缺损。最初，由于动物处理过程中软骨片的物理破坏，制造出结构良好的新气管的成功率受到阻碍，但到研究结束时，18个新气管中有15个(83.3%)结构良好。在15个结构良好的新气管中，11个用于节段性重建，放置时间长达21天。组织学检查显示存在数量不等的存活上皮、带血管的颈阔肌瓣、一层藏红素O阳性的软骨层以及软骨内骨化的证据。接受节段性重建的兔显示出良好的气管整合，有一个有血管支撑的可存活的上皮，当被触诊时，软骨足够坚固，可以维持管腔。结果表明，可以从自体细胞构建有活力的三层无支架新气管，并可以整合到自然气管中修复节段性缺损。
The repair of large tracheal segmental defects remains an unsolved problem. The goal of this study is to apply tissue engineering principles for the fabrication of large segmental trachea replacements. Engineered tracheal replacements composed of autologous cells (“neotracheas”) were tested in a New Zealand White rabbit model. Neotracheas were formed in the rabbit neck by wrapping a silicone tube with consecutive layers of skin epithelium, platysma muscle and an engineered cartilage sheet, and allowing the construct to mature for 8–12 weeks. In total, 28 rabbits were implanted and the neotracheas assessed for tissue morphology. In 11 cases, neotracheas deemed sufficiently strong were used to repair segmental tracheal defects. Initially, the success rate of producing structurally sound neotracheas was impeded by physical disruption of the cartilage sheets during animal handling, but by the end of the study, 15 of 18 neotracheas (83.3%) were structurally sound. Of the 15 structurally sound neotracheas, 11 were used for segmental reconstruction and were left in place for up to 21 days. Histological examination showed the presence of variable amounts of viable epithelium, a vascularized platysma flap, and a layer of safranin O-positive cartilage along with evidence of endochondral ossification. Rabbits that had undergone segmental reconstruction showed good tracheal integration, had a viable epithelium with vascular support, and the cartilage was sufficiently strong to maintain a lumen when palpated. The results demonstrated that viable, tri-layered, scaffold-free neotracheas could be constructed from autologous cells and could be integrated into native trachea to repair a segmental defect.
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影响因子：
3.6
作者：
ERJEFALT, JS;ERJEFALT, I;PERSSON, CGA
通讯作者：
PERSSON, CGA
兔带蒂组织工程气管对节段性气管缺损的长期功能重建。
影响因子：
14
作者：
Luo, Xusong;Liu, Yi;Zhou, Guangdong
通讯作者：
Zhou, Guangdong
影响因子：
2.6
作者：
LLOYD, DC;TAYLOR, PM
通讯作者：
TAYLOR, PM
影响因子：
4.2
作者：
Igai, Hitoshi;Chang, Sung Soo;Yokomise, Hiroyasu
通讯作者：
Yokomise, Hiroyasu
影响因子：
168.9
作者：
Macchiarini, Paolo;Jungebluth, Philipp;Birchall, Martin A.
通讯作者：
Birchall, Martin A.