Roles of insulin-like growth factors and their binding proteins in the differentiation of mouse tongue myoblasts.
Roles of insulin-like growth factors and their binding proteins in the differentiation of mouse tongue myoblasts.
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胰岛素样生长因子及其结合蛋白在小鼠舌成肌细胞分化中的作用。
DOI：
10.1387/ijdb.12382947
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
T. Diekwisch
中科院分区：
文献类型：
--
作者：
A. Yamane;T. Urushiyama;T. Diekwisch
为了研究胰岛素样生长因子（IGFs）及其结合蛋白（igfbp）在舌成肌细胞分化中的作用，我们建立了小鼠舌器官培养系统，并检测了外源性IGF-I、外源性IGFBP4、5、6和des(1-3)IGF-I（一种对igfbp亲和力降低的IGF-I类似物）对舌成肌细胞分化的影响。外源性IGF-I刺激舌成肌细胞分化，诱导内源性IGFBP4、5、6的表达，提示这些igfbp参与了IGF-I对舌成肌细胞分化的调控。外源性IGFBP4和igfbp5轻微刺激舌成肌细胞的早期分化，其中肌生成素参与其中。这种刺激似乎是由于这些igfbp与内源性IGFs结合，保护内源性IGFs免受蛋白水解降解。低浓度的des(1-3)IGF-I刺激舌成肌细胞分化，而高浓度的des(1-3)IGF-I则抑制舌成肌细胞分化。高浓度des(1-3)IGF-I处理后舌形异常，细胞密度低，苏木精和伊红染色强度低，提示这种抑制是由于高浓度des(1-3)IGF-I引起舌组织对毒性的异常反应。根据这些结果，我们认为igfbp可能通过将游离IGFs的浓度控制在一个适合成肌细胞分化进程的范围内来调节小鼠舌成肌细胞的分化。
To study the roles of insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) in the differentiation of tongue myoblasts, we established a mouse tongue organ culture system and examined the effects of exogenous IGF-I, exogenous IGFBP4, 5, 6, and des(1-3)IGF-I, an IGF-I analogue with reduced affinity for IGFBPs, on the differentiation of tongue myoblasts. The exogenous IGF-I stimulated differentiation of tongue myoblasts and induced the expressions of endogenous IGFBP4, 5, and 6, suggesting that these IGFBPs were involved in the regulation of tongue myoblast differentiation by the IGF-I. Exogenous IGFBP4 and 5 slightly stimulated early tongue myoblast differentiation in which myogenin was involved. The stimulation seems to be due to the protection of endogenous IGFs from proteolytic degradation by the binding of these IGFBPs to endogenous IGFs. A low concentration of des(1-3)IGF-I stimulated tongue myoblast differentiation, whereas high concentrations of des(1-3)IGF-I inhibited it. The abnormal shape of the tongue, low cell density and low staining intensity with hematoxylin and eosin in tongues treated with high concentrations of des(1-3)IGF-I, suggest that the inhibition is due to abnormal reactions of tongue tissues to the toxicity caused by high concentrations of des(1-3)IGF-I. From these results, we suggest that IGFBPs may function to regulate the differentiation of mouse tongue myoblasts by controlling the concentration of free IGFs within a range suitable for the progress of tongue myoblast differentiation.
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胰岛素样生长因子 1 或成纤维细胞生长因子 2 抑制骨骼肌分化需要丝裂原激活蛋白激酶激酶 (MEK) 活性。
DOI：
10.1210/endo.139.4.5950
发表时间：
1998
期刊：
Endocrinology.
影响因子：
--
作者：
Weyman,CM;Wolfman,A
通讯作者：
Wolfman,A
DOI：
10.1016/s0021-9258(18)98496-6
发表时间：
1991-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Florini;K. Magri;D. Ewton;P. James;K. Grindstaff;P. Rotwein
通讯作者：
J. Florini;K. Magri;D. Ewton;P. James;K. Grindstaff;P. Rotwein
影响因子：
20.3
作者：
John I. Jones;D. Clemmons
通讯作者：
John I. Jones;D. Clemmons
DOI：
10.1387/ijdb.10213080
发表时间：
1999
期刊：
The International journal of developmental biology
影响因子：
--
作者：
Kirsten R. Dalrymple;Theodore I. Prigozy;Mark Mayo;Lawrence H. Kedes;C. F. Shuler
通讯作者：
Kirsten R. Dalrymple;Theodore I. Prigozy;Mark Mayo;Lawrence H. Kedes;C. F. Shuler
细胞因子对体外肌肉细胞分泌胰岛素样生长因子结合蛋白的影响。
影响因子：
4.8
作者：
McCusker,RH;Clemmons,DR
通讯作者：
Clemmons,DR