Contrast-enhanced microCT evaluation of degeneration following partial and full width injuries to the mouse lumbar intervertebral disc.
Contrast-enhanced microCT evaluation of degeneration following partial and full width injuries to the mouse lumbar intervertebral disc.
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DOI：
10.1038/s41598-022-19487-9
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发表时间：
2022-09-16
影响因子：
4.6
通讯作者：
Gupta, Munish C.
中科院分区：
文献类型：
--
作者：
Walk, Remy E.;Moon, Hong Joo;Tang, Simon Y.;Gupta, Munish C.
小鼠椎间盘的靶向损伤(IVD)经常被用来概括人类病理的退行性级联反应。由于损伤的大小和位置可能不同，因此检查不同损伤对IVD变性的影响是至关重要的。因此，我们使用增强的显微计算机断层扫描和组织学分析，评估了小鼠腰椎IVD的部分或全部损伤所导致的退变进展。采用腹膜后外侧入路入路，切开一侧纤维环或刺破双侧纤维环，造成腰椎IVD损伤。本研究选用3~4月龄雌性C57BL/6J小鼠。他们被分成三组，分别接受半宽、全宽或假损伤。手术暴露L5/6和L6/S1静脉畸形，然后用手术刀(半宽)或33G针(全宽)损伤L6/S1静脉畸形，L5/6作为内对照。这些动物在手术后2周、4周或8周恢复并安乐死以进行评估。用对比增强显微CT(CE？CT)和组织学分析评估IVD的退变情况。高分辨率3D CE？CT对IVD的评估证实，各自的损伤位于纤维环的一侧或横跨IVD的整个宽度。2周后，全宽度损伤导致髓核、纤维环和界面明显恶化，并持续到后8周，而部分宽度损伤导致局限性破坏，仅限于纤维环。使用CEµCT显示了部分和全宽损伤引起的明显的IVD退变轮廓。部分宽度损伤可作为局限性纤维环损伤所致IVD变性的另一种模型。
A targeted injury to the mouse intervertebral disc (IVD) is often used to recapitulate the degenerative cascade of the human pathology. Since injuries can vary in magnitude and localization, it is critical to examine the effects of different injuries on IVD degeneration. We thus evaluated the degenerative progression resulting from either a partial- or full-width injury to the mouse lumbar IVD using contrast-enhanced micro-computed tomography and histological analyses. A lateral-retroperitoneal surgical approach was used to access the lumbar IVD, and the injuries to the IVD were produced by either incising one side of the annulus fibrosus or puncturing both sides of the annulus fibrosus. Female C57BL/6J mice of 3–4 months age were used in this study. They were divided into three groups to undergo partial-width, full-width, or sham injuries. The L5/6 and L6/S1 lumbar IVDs were surgically exposed, and then the L6/S1 IVDs were injured using either a surgical scalpel (partial-width) or a 33G needle (full-width), with the L5/6 serving as an internal control. These animals recovered and then euthanized at either 2-, 4-, or 8-weeks after surgery for evaluation. The IVDs were assessed for degeneration using contrast-enhanced microCT (CEµCT) and histological analysis. The high-resolution 3D CEµCT evaluation of the IVD confirmed that the respective injuries were localized within one side of the annulus fibrosus or spanned the full width of the IVD. The full-width injury caused significant deteriorations in the nucleus pulposus, annulus fibrous and at the interfaces after 2 weeks, which was sustained through the 8 weeks, while the partial width injury caused localized disruptions that remained limited to the annulus fibrosus. The use of CEµCT revealed distinct IVD degeneration profiles resulting from partial- and full-width injuries. The partial width injury may serve as an alternative model for IVD degeneration resulting from localized annulus fibrosus injuries.
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影响因子：
5.6
作者：
Shi, Changgui;Das, Vaskar;Im, Hee-Jeong
通讯作者：
Im, Hee-Jeong
影响因子：
3.7
作者：
Melgoza IP;Chenna SS;Tessier S;Zhang Y;Tang SY;Ohnishi T;Novais EJ;Kerr GJ;Mohanty S;Tam V;Chan WCW;Zhou CM;Zhang Y;Leung VY;Brice AK;Séguin CA;Chan D;Vo N;Risbud MV;Dahia CL
通讯作者：
Dahia CL
DOI：
10.1097/phm.0000000000000818
发表时间：
2018-03
影响因子：
3
作者：
Tian Z;Ma X;Yasen M;Mauck RL;Qin L;Shofer FS;Smith LJ;Pacifici M;Enomoto-Iwamoto M;Zhang Y
通讯作者：
Zhang Y
影响因子：
2.8
作者：
Berger-Roscher, Nikolaus;Galbusera, Fabio;Wilke, Hans-Joachim
通讯作者：
Wilke, Hans-Joachim
影响因子：
4.5
作者：
Rao, Raj D.;Bagaria, Vaibhav B.;Cooley, Brian C.
通讯作者：
Cooley, Brian C.