Time-Resolved and Comprehensive Analysis of Surface Glycoproteins Reveals Distinct Responses of Monocytes and Macrophages to Bacterial Infection.
Time-Resolved and Comprehensive Analysis of Surface Glycoproteins Reveals Distinct Responses of Monocytes and Macrophages to Bacterial Infection.
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DOI：
10.1002/anie.202102692
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发表时间：
2021-05-10
期刊：
影响因子：
--
通讯作者：
Wu R
中科院分区：
文献类型：
--
作者：
Suttapitugsakul S;Tong M;Wu R
免疫细胞表面的糖蛋白在对病原体的应答中起着极其重要的作用。然而，对免疫应答过程中的表面糖蛋白进行系统和时间分辨的研究仍有待探索。结合表面糖蛋白的选择性浓缩和多重蛋白质组学，我们全局和定点定量了THP-1单核细胞和巨噬细胞在细菌感染和单核细胞向巨噬细胞分化过程中表面糖蛋白的动态变化。时间分辨分析揭示了两种细胞表面糖蛋白的瞬时变化和差异重塑，以及受调控的糖蛋白的潜在上游调节和下游影响。此外，我们还发现了新的参与免疫反应的表面糖蛋白，如APMAP，以及糖蛋白的位点特异性变化。这项研究提供了前所未有的信息，加深了我们对糖蛋白和细胞活动的理解。
Glycoproteins on the surface of immune cells play extremely important roles in response to pathogens. Yet, a systematic and time-resolved investigation of surface glycoproteins during the immune response remains to be explored. Integrating selective enrichment of surface glycoproteins with multiplexed proteomics, we globally and site-specifically quantified the dynamics of surface glycoproteins on THP-1 monocytes and macrophages in response to bacterial infection and during the monocyte-to-macrophage differentiation. The time-resolved analysis reveals transient changes and differential remodeling of surface glycoproteins on both cell types, and potential upstream regulators and downstream effects of the regulated glycoproteins. Besides, we identified novel surface glycoproteins participating in the immune response such as APMAP, and site-specific changes of glycoproteins. This study provides unprecedented information to deepen our understanding of glycoproteins and cellular activities.
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影响因子：
7.7
作者：
Cani, Patrice D.;Amar, Jacques;Burcelin, Remy
通讯作者：
Burcelin, Remy
影响因子：
6.1
作者：
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通讯作者：
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影响因子：
3.7
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DOI：
10.4049/jimmunol.1102695
发表时间：
2012-06-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Masopust D;Picker LJ
通讯作者：
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DOI：
10.1002/ar.20757
发表时间：
2010-06
期刊：
Anatomical record (Hoboken, N.J. : 2007)
影响因子：
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作者：
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通讯作者：
Park PW