Immunological Study of Combined Administration of SARS-CoV-2 DNA Vaccine and Inactivated Vaccine.
Immunological Study of Combined Administration of SARS-CoV-2 DNA Vaccine and Inactivated Vaccine.
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SARS-COV-2 DNA疫苗和灭活疫苗联合给药的免疫学研究。
DOI：
10.3390/vaccines10060929
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发表时间：
2022-06-10
期刊：
影响因子：
7.8
通讯作者：
Li, Qihan
中科院分区：
文献类型：
--
作者：
Meng, Ziyan;Ma, Danjing;Duan, Suqin;Zhang, Jingjing;Yue, Rong;Li, Xinghang;Gao, Yang;Li, Xueqi;Zeng, Fengyuan;Xu, Xiangxiong;Jiang, Guorun;Liao, Yun;Fan, Shengtao;Niu, Zhenye;Li, Dandan;Yu, Li;Zhao, Heng;Xu, Xingli;Wang, Lichun;Zhang, Ying;Liu, Longding;Li, Qihan
目的：我们构建了两种含有多个SARS-CoV-2变异体受体结合域（RBD）基因的DNA疫苗，并将其与灭活疫苗以各种不同的方案联合使用，以探索潜在的针对SARS-CoV-2变异体的新型免疫策略。研究方法：使用了具有不同SARS-CoV-2毒株（武汉-Hu-1、B.1.351、B.1.617.2、C.37）的不同信号肽（即分泌型和膜信号肽）和RBD蛋白基因的两种DNA疫苗候选物。试验了4种不同的DNA疫苗和灭活疫苗组合，即A组：3针DNA疫苗; B组：3针DNA疫苗和1针灭活疫苗; C组：2针灭活疫苗和1针DNA疫苗; D组：DNA疫苗和灭活疫苗共接种2针。根据使用的信号肽对亚组进行分组（亚组1含有分泌的信号肽，亚组2含有膜信号肽）。评价DNA疫苗的体外表达、免疫小鼠的体液和细胞免疫应答、局部淋巴结中免疫细胞群的变化以及血清样品中促炎细胞因子水平。结果如下：A组中所有SARS-CoV-2毒株的抗体应答和细胞免疫均较弱;对于B组，针对B.1.617.2变异株的中和抗体（Na B）滴度显著增强。C组抗体应答明显增加（C1组和C2组针对武汉-Hu-1毒株的NAb滴度分别为768和1154，而C2组为576）和细胞免疫应答，特别是变异体B.1.617.2（C1组和C2组分别为3240（p < 0.001）和2430（p < 0.05），而对照组为450）; D组显示免疫原性改善。C组诱导多种细胞因子水平升高。结论：我们构建的候选DNA疫苗作为加强剂施用，可以增强针对COVID-19的灭活疫苗的体液和细胞免疫应答，特别是针对B.1.617.2的灭活疫苗。
Objective: We constructed two DNA vaccines containing the receptor-binding domain (RBD) genes of multiple SARS-CoV-2 variants and used them in combination with inactivated vaccines in a variety of different protocols to explore potential novel immunization strategies against SARS-CoV-2 variants. Methods: Two DNA vaccine candidates with different signal peptides (namely, secreted and membrane signal peptides) and RBD protein genes of different SARS-CoV-2 strains (Wuhan-Hu-1, B.1.351, B.1.617.2, C.37) were used. Four different combinations of DNA and inactivated vaccines were tested, namely, Group A: three doses of DNA vaccine; B: three doses of DNA vaccine and one dose of inactivated vaccine; C: two doses of inactivated vaccine and one dose of DNA vaccine; and D: coadministration of DNA and inactivated vaccines in two doses. Subgroups were grouped according to the signal peptide used (subgroup 1 contained secreted signal peptides, and subgroup 2 contained membrane signal peptides). The in vitro expression of the DNA vaccines, the humoral and cellular immunity responses of the immunized mice, the immune cell population changes in local lymph nodes, and proinflammatory cytokine levels in serum samples were evaluated. Results: The antibody responses and cellular immunity in Group A were weak for all SARS-CoV-2 strains; for Group B, there was a great enhancement of neutralizing antibody (Nab) titers against the B.1.617.2 variant strain. Group C showed a significant increase in antibody responses (NAb titers against the Wuhan-Hu-1 strain were 768 and 1154 for Group C1 and Group C2, respectively, versus 576) and cellular immune responses, especially for variant B.1.617.2 (3240 (p < 0.001) and 2430 (p < 0.05) for Group C1 and Group C2, versus 450); Group D showed an improvement in immunogenicity. Group C induced higher levels of multiple cytokines. Conclusion: The DNA vaccine candidates we constructed, administered as boosters, could enhance the humoral and cellular immune responses of inactivated vaccines against COVID-19, especially for B.1.617.2.
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影响因子：
5.5
作者：
Dey A;Chozhavel Rajanathan TM;Chandra H;Pericherla HPR;Kumar S;Choonia HS;Bajpai M;Singh AK;Sinha A;Saini G;Dalal P;Vandriwala S;Raheem MA;Divate RD;Navlani NL;Sharma V;Parikh A;Prasath S;Sankar Rao M;Maithal K
通讯作者：
Maithal K
影响因子：
12.7
作者：
Fernandez, Jorge;Bruneau, Nicole;Ramirez, Eugenio
通讯作者：
Ramirez, Eugenio
DOI：
10.1056/nejmoa2035389
发表时间：
2021-02-04
期刊：
The New England journal of medicine
影响因子：
--
作者：
Baden LR;El Sahly HM;Essink B;Kotloff K;Frey S;Novak R;Diemert D;Spector SA;Rouphael N;Creech CB;McGettigan J;Khetan S;Segall N;Solis J;Brosz A;Fierro C;Schwartz H;Neuzil K;Corey L;Gilbert P;Janes H;Follmann D;Marovich M;Mascola J;Polakowski L;Ledgerwood J;Graham BS;Bennett H;Pajon R;Knightly C;Leav B;Deng W;Zhou H;Han S;Ivarsson M;Miller J;Zaks T;COVE Study Group
通讯作者：
COVE Study Group
影响因子：
7.3
作者：
Lee LYY;Izzard L;Hurt AC
通讯作者：
Hurt AC
DOI：
10.1016/j.ijbiomac.2021.08.076
发表时间：
2021-10-01
影响因子：
8.2
作者：
Fathizadeh H;Afshar S;Masoudi MR;Gholizadeh P;Asgharzadeh M;Ganbarov K;Köse Ş;Yousefi M;Kafil HS
通讯作者：
Kafil HS