Molecular identification and population differentiation of Aurelia spp. ephyrae in sea cucumber aquaculture ponds of northern China
Molecular identification and population differentiation of Aurelia spp. ephyrae in sea cucumber aquaculture ponds of northern China
复制标题
Aurelia spp 的分子鉴定和群体分化。
DOI：
10.1007/s00343-020-0022-9
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发表时间：
2020-10
影响因子：
1.6
通讯作者：
Dong Zhijun
中科院分区：
文献类型：
--
作者：
Peng Saijun;Liu Qingqing;Wang Lei;Sun Tingting;Shiganova Tamara;Dong Zhijun
据报道，海月水母属碟状幼体在渤海和黄海的海参养殖池塘中形成水华。为了确定其种类，我们基于线粒体16S rRNA基因对海月水母属碟状幼体和成体水母进行了遗传分析。在海参养殖池塘采集了4个海月水母属碟状幼体种群的样本，在沿海水域采集了4个海月水母属成体水母种群的样本。通过BLASTn搜索，我们发现养殖池塘中采集的碟状幼体和沿海水域采集的成体水母都属于海月水母。从16S rRNA基因中获得了17个单倍型。166个海月水母个体的总体单倍型多样性和核苷酸多样性分别为0.686%和0.329%，表明单倍型多样性较高，核苷酸多样性较低。此外，在采样点相近的情况下，碟状幼体种群的单倍型多样性通常低于成体水母种群。海参养殖池塘中采集的碟状幼体种群与沿海水域采集的海月水母成体水母之间的遗传分化不显著，这表明海参养殖池塘中的碟状幼体种群与沿海水域中的成体水母种群属于同一遗传群体。对16S rRNA区域的系统地理分析表明，单倍型与种群的地理分布之间没有显著相关性。成对固定指数值显示潍坊的海月水母种群与其他地区之间存在显著的遗传分化和有限的基因流。
Aurelia spp. ephyrae have been reported to form blooms in sea cucumber aquaculture ponds in the Bohai and Yellow Seas. To identify the species, we carried out a genetic analysis of Aurelia spp. ephyrae and medusae based on mitochondrial 16S rRNA gene. Samples of four Aurelia sp. ephyrae populations were collected in sea cucumber aquaculture ponds and samples of four Aurelia sp. medusae populations were collected in coastal waters. Using a BLASTn search, we found that both the ephyrae collected in the aquaculture ponds and medusae collected in coastal waters belong to Aurelia coerulea. Seventeen haplotypes were recovered from the 16S rRNA gene. The overall haplotype diversity and nucleotide diversity of the 166 A. coerulea individuals were 0.686% and 0.329%, respectively, indicating high haplotype diversity and low nucleotide diversity. Moreover, the haplotype diversity of ephyrae populations were generally lower than that of medusae populations with close sampling points. The genetic differentiation between ephyrae populations collected in the sea cucumber aquaculture ponds and A. coerulea medusae collected in coastal waters was not significant, suggesting the ephyrae populations in the sea cucumber culture ponds were part of the same genetic group as the medusae populations in the coastal waters. Phylogeographic analysis of the 16S rRNA region revealed that there was no significant correlation between the haplotypes and the geographic distribution of populations. Pairwise fixation index values showed significant genetic differentiation and limited gene fl ow between A. coerulea population of Weifang and other locations.
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DOI：
--
发表时间：
2002
期刊：
--
影响因子：
--
作者：
通讯作者：
--
影响因子：
2
作者：
D. Whitmore;T. Thai;C. Craft
通讯作者：
D. Whitmore;T. Thai;C. Craft
影响因子：
4.1
作者：
Katja Stopar;A. Ramšak;P. Trontelj;A. Malej
通讯作者：
Katja Stopar;A. Ramšak;P. Trontelj;A. Malej
影响因子：
0.6
作者：
S. Uye
通讯作者：
S. Uye
影响因子：
2.6
作者：
Lucas, CH
通讯作者：
Lucas, CH