Can alternative epitope mapping approaches increase the impact of B-cell epitopes in food allergy diagnostics?
Can alternative epitope mapping approaches increase the impact of B-cell epitopes in food allergy diagnostics?
复制标题
DOI：
10.1111/cea.13291
复制
发表时间：
2019-01
期刊：
影响因子：
--
通讯作者：
Otten HG
中科院分区：
文献类型：
--
作者：
Ehlers AM;Blankestijn MA;Knulst AC;Klinge M;Otten HG
体外过敏诊断目前基于检测完整过敏原或其混合物上的特异性IgE结合。这种方法有缺点，因为它可能产生假阴性和/或假阳性结果。因此，我们回顾了已知的食物过敏原B细胞表位对预测短暂性或持久性、耐受性或过敏以及过敏反应严重程度的影响，并研究了新的表位定位策略，旨在改善基于血清的过敏诊断。最近的表位定位方法在表位鉴定中有价值，并且在某些情况下可以通过使用过敏患者主要识别的表位来增加过敏诊断的特异性。然而，到目前为止，这些方法并没有导致临床相关和不相关表位的区分，因为多克隆血清IgE结合表位谱似乎过于个体，与患者的疾病状态无关。新的表位定位策略是克服这些障碍所必需的。使用患者衍生的单克隆抗体代替患者血清，对临床相关和不相关的表位组合进行功能表征，以其诱导脱颗粒的能力来区分，可能是一种有希望的方法，可以更深入地了解过敏反应，并改善基于血清的过敏诊断。
In vitro allergy diagnostics are currently based on the detection of specific IgE binding on intact allergens or a mixture thereof. This approach has drawbacks as it may yield false‐negative and/or false‐positive results. Thus, we reviewed the impact of known B‐cell epitopes of food allergens to predict transience or persistence, tolerance or allergy and the severity of an allergic reaction and to examine new epitope mapping strategies meant to improve serum‐based allergy diagnostics. Recent epitope mapping approaches have been worthwhile in epitope identification and may increase the specificity of allergy diagnostics by using epitopes predominately recognized by allergic patients in some cases. However, these approaches did not lead to discrimination between clinically relevant and irrelevant epitopes so far, since the polyclonal serum IgE‐binding epitope spectrum seems to be too individual, independent of the disease status of the patients. New epitope mapping strategies are necessary to overcome these obstacles. The use of patient‐derived monoclonal antibodies instead of patient sera for functional characterization of clinically relevant and irrelevant epitope combinations, distinguished by their ability to induce degranulation, might be a promising approach to gain more insight into the allergic reaction and to improve serum‐based allergy diagnostics.
登录
查看更多内容
影响因子：
3.6
作者：
Bogh, K. L.;Nielsen, H.;Roggen, E. L.
通讯作者：
Roggen, E. L.
影响因子：
14.2
作者：
Cocco, RR;Järvinen, KM;Beyer, K
通讯作者：
Beyer, K
影响因子：
14.2
作者：
Berkowska, Magdalena A.;Heeringa, Jorn J.;van Zelm, Menno C.
通讯作者：
van Zelm, Menno C.
影响因子：
3.7
作者：
Chen JB;Yang YH;Lee WC;Liou CW;Lin TK;Chung YH;Chuang LY;Yang CH;Chang HW
通讯作者：
Chang HW
影响因子：
14.2
作者：
Davies, Janet M.;Platts-Mills, Thomas A.;Aalberse, Rob C.
通讯作者：
Aalberse, Rob C.