Induction of metallothionein and other mRNA species by carcinogens and tumor promoters in primary human skin fibroblasts
Induction of metallothionein and other mRNA species by carcinogens and tumor promoters in primary human skin fibroblasts
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致癌物和肿瘤促进剂在原代人皮肤成纤维细胞中诱导金属硫蛋白和其他 mRNA 种类
DOI：
10.1128/mcb.6.5.1760-1766.1986
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发表时间：
1986
影响因子：
5.3
通讯作者：
Peter Herrlich
中科院分区：
文献类型：
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作者：
Peter Angel;Annette Poting;U. Mallick;H. Rahmsdorf;M. Schorpp;Peter Herrlich
我们使用核酸杂交和 cDNA 克隆技术来分离响应肿瘤启动子 12-O-十四烷酰佛波醇-13-乙酸酯 (TPA) 的人类序列。这些克隆被用作探针来检查在指数生长的原代人类成纤维细胞的增殖被抑制后发生的基因表达的变化。通过用紫外光、丝裂霉素 C、TPA 或紫外光诱导的细胞外蛋白质合成诱导因子 EPIF 处理细胞，这些探针检测到的转录物水平得到协调增加（M. Schorpp、U. Mallick、H. J. Rahmsdorf 和 P. Herrlich，Cell 37：861-868，1984）。这些转录本编码的蛋白质通过杂交促进翻译和 cDNA 测序进行了表征。一个 cDNA 克隆与金属硫蛋白 IIa 基因同源，一组相关克隆选择分泌的 TPA 诱导蛋白 XHF1 的 RNA (U. Mallick, H. J. Rahmsdorf, N. Yamamoto, H. Ponta, R.-D. Wegner, and P. Herrlich, Proc. Natl. Acad. Sci. USA 79:7886-7890， 1982）。
We used nucleic acid hybridization and cDNA cloning techniques to isolate human sequences that respond to the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). These clones were used as probes to examine changes of gene expression that occurred after the proliferation of exponentially growing primary human fibroblasts was arrested. Transcript levels detected by these probes were increased coordinately by treatment of the cells with UV light, mitomycin C, TPA, or the UV light-induced extracellular protein synthesis-inducing factor EPIF (M. Schorpp, U. Mallick, H. J. Rahmsdorf, and P. Herrlich, Cell 37:861-868, 1984). Proteins coded for by these transcripts were characterized by hybrid-promoted translation and by cDNA sequencing. One of the cDNA clones was homologous to the metallothionein IIa gene, and one set of related clones selected RNA for the secreted TPA-inducible protein XHF1 (U. Mallick, H. J. Rahmsdorf, N. Yamamoto, H. Ponta, R.-D. Wegner, and P. Herrlich, Proc. Natl. Acad. Sci. USA 79:7886-7890, 1982).
培养小鼠细胞特定 mRNA 的生长相关变化。
DOI：
10.1073/pnas.80.14.4271
发表时间：
1983
影响因子：
11.1
作者：
Linzer,DI;Nathans,D
通讯作者：
Nathans,D
DOI：
10.1073/pnas.79.6.2018
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
EISINGER, M;MARKO, O
通讯作者：
MARKO, O