Effectiveness of deferoxamine on ferric chloride-induced epilepsy in rats
Effectiveness of deferoxamine on ferric chloride-induced epilepsy in rats
复制标题
去铁胺对氯化铁所致大鼠癫痫的疗效
DOI：
10.1016/j.brainres.2017.01.001
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发表时间：
2017-03
期刊：
影响因子：
2.9
通讯作者：
Chen Liang
中科院分区：
文献类型：
--
作者：
Zou Xiang;Jiang Shize;Wu Zehan;Shi Yimin;Cai Shengyong;Zhu Renqing;Chen Liang
铁超载被认为是出血性中风后难治性癫痫的常见原因。本研究旨在探讨铁螯合剂去铁胺（DFO）对三氯化铁诱导的癫痫大鼠模型的潜在癫痫控制作用。将24只大鼠随机分为4组：I组为空白对照组，II组为假手术组，III组为癫痫组，IV组为癫痫组。DFO干预组每日腹腔注射DFO 100 mg/kg，连续14 d。通过行为学研究、脑电图（EEG）、磁共振成像（MRI）扫描和组织分析评估结局。根据行为学观察和脑电图分析，DFO干预后癫痫明显受到抑制。脑皮质铁含量减少，表现为T2-MRI图像上低信号区减少（p = 0.006）和组织分析（p < 0.001），同时超氧化物歧化酶（SOD）活性增加（p < 0.001）。Western blot分析表明DFO处理后局部转铁蛋白含量下降。DFO能有效地清除铁离子，从而有助于癫痫的控制。这一发现暗示了DFO在出血性卒中后难治性癫痫患者中的潜在治疗价值。
Iron overload has been regarded as a common cause for refractory epilepsies in patients after hemorrhagic strokes. This study is to examine the potential epilepsy control effect of deferoxamine (DFO), an iron chelator, on a ferric chloride-induced epilepsy rat model. Twenty four rats were divided into 4 groups: group I is blank control group, group II is sham group with intracortical injection of saline, group III is epilepsy group with intracortical injection of iron and saline treatment, group IV is treatment group with intracortical injection of iron and DFO treatment. For the DFO intervention group, a daily dose of 100 mg/kg DFO via peritoneal injection was applied for 14 days. Outcomes were evaluated by behavioral study, electroencephalography (EEG), magnetic resonance imaging (MRI) scan and tissue analysis. Epilepsies according to behavioral observations and EEG analysis were significantly suppressed after intervention of DFO. Reduction of iron content in the brain cortex was proved by diminished low signal area on T2-MRI images (p = 0.006) and tissue analysis (p < 0.001), simultaneously the superoxide dismutase (SOD) activity increased (p < 0.001). Western blot analysis demonstrated the decreasing of local transferrin after DFO treatment. DFO is efficient at Fe clearance, thus helpful in epilepsy control. This finding implies potential therapeutic value of DFO in patients with refractory epilepsy after hemorrhagic stroke.
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影响因子：
2.2
作者：
Mishra, Monika;Singh, Rameshwar;Sharma, Deepak
通讯作者：
Sharma, Deepak
DOI：
10.1007/bf02964604
发表时间：
1990-04
期刊：
The Pavlovian Journal of Biological Science
影响因子：
--
作者：
A. Mori;M. Hiramatsu;I. Yokoi;R. Edamatsu
通讯作者：
A. Mori;M. Hiramatsu;I. Yokoi;R. Edamatsu
影响因子：
9.9
作者：
L. Willmore;J. J. Rubin-J.
通讯作者：
L. Willmore;J. J. Rubin-J.
影响因子：
8.3
作者：
Okauchi M;Hua Y;Keep RF;Morgenstern LB;Schallert T;Xi G
通讯作者：
Xi G
影响因子：
2.9
作者：
L. Willmore;W. Triggs;J. Gray
通讯作者：
L. Willmore;W. Triggs;J. Gray