An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination.
An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination.
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DOI：
10.1016/j.jim.2014.10.012
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发表时间：
2015-01
影响因子：
2.2
通讯作者：
Butter C
中科院分区：
文献类型：
--
作者：
Ruiz-Hernandez R;Peroval M;Boyd A;Balkissoon D;Staines K;Smith A;Butter C
更好地了解鸡对流感病毒的免疫反应对于制定新的疫苗接种和控制策略至关重要。我们开发了一种方法，将感染的鸡肾细胞（CKC）与脾细胞一起培养，用于IFNγ ELISpot测定，以计数针对流感病毒抗原的离体应答。来自用流感攻击的鸟类的脾细胞显示出对流感病毒的特异性应答，应答细胞主要为CD8阳性。该检测方法还可用于检测重组鸡痘病毒载体流感病毒核蛋白和基质蛋白免疫鸡后产生IFNγ细胞的抗原特异性增强。感染禽流感的鸡产生IFNγ应答。在ELISpot中使用感染的CKC克服了检测应答的局限性。该方法允许定量流感特异性CD8 T细胞。使用重组病毒感染CKC可以进一步确定抗原特异性。
A better understanding of the immune responses of chickens to the influenza virus is essential for the development of new strategies of vaccination and control. We have developed a method incorporating infected chicken kidney cells (CKC) in culture with splenocytes in an IFNγ ELISpot assay to enumerate ex vivo responses against influenza virus antigens. Splenocytes from birds challenged with influenza showed specific responses to the influenza virus, with responding cells being mainly CD8 positive. The utility of the assay was also demonstrated in the detection of an antigen specific enhancement of IFNγ producing cells from birds vaccinated with recombinant Fowlpox vectored influenza nucleoprotein and matrix protein. Chickens infected with avian influenza developed IFNγ responses. The use of infected CKC in ELISpot overcomes limitations at detection of responses. This methods allows the quantification of influenza specific CD8 T cells. The use of recombinant virus to infect CKC can further define antigen specificity.
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影响因子：
5.5
作者：
Boyd, Amy C.;Ruiz-Hernandez, Raul;Peroval, Marylene Y.;Carson, Connor;Balkissoon, Devanand;Staines, Karen;Turner, Alison V.;Hill, Adrian V. S.;Gilbert, Sarah C.;Butter, Colin
通讯作者：
Butter, Colin
影响因子：
3.7
作者：
Reemers SS;van Haarlem DA;Sijts AJ;Vervelde L;Jansen CA
通讯作者：
Jansen CA
DOI：
10.1093/cid/cis327
发表时间：
2012-07
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Lillie PJ;Berthoud TK;Powell TJ;Lambe T;Mullarkey C;Spencer AJ;Hamill M;Peng Y;Blais ME;Duncan CJ;Sheehy SH;Havelock T;Faust SN;Williams RL;Gilbert A;Oxford J;Dong T;Hill AV;Gilbert SC
通讯作者：
Gilbert SC
DOI：
10.1128/aem.16.4.588-594.1968
发表时间：
1968-01-01
期刊：
APPLIED MICROBIOLOGY
影响因子：
--
作者：
GAUSH, CR;SMITH, TF
通讯作者：
SMITH, TF
影响因子：
5.4
作者：
Oh, S;McCaffery, JM;Eichelberger, MC
通讯作者：
Eichelberger, MC