5-Hydroxymethylcytosine expression in metastatic melanoma versus nodal nevus in sentinel lymph node biopsies.
5-Hydroxymethylcytosine expression in metastatic melanoma versus nodal nevus in sentinel lymph node biopsies.
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哨兵淋巴结活检中的5-羟基甲基胞嘧啶在转移性黑色素瘤与淋巴结中的表达。
DOI：
10.1038/modpathol.2014.99
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发表时间：
2015-02
期刊：
影响因子：
--
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中科院分区：
文献类型：
--
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前哨淋巴结活检是对新诊断的黑色素瘤患者进行分期，这些黑色素瘤具有赋予确定转移潜力水平的组织病理学属性。由于良性痣细胞也可能在淋巴结中形成“沉积物”（结痣），前哨淋巴结内转移性黑色素瘤的病理学评价可能具有挑战性。从Brigham and Women's Hospital Department of Pathology（2011-2014）的档案中检索了28例包含转移性黑色素瘤（N=18）或淋巴结痣（N=10）的前哨淋巴结活检病例。此外，还纳入了2例前哨淋巴结病例，这些病例倾向于代表转移性疾病，但其组织病理学特征被视为不明确，倾向于黑色素瘤。使用免疫组织化学和/或直接免疫荧光对所有病例进行黑素细胞谱系标记物MART-1和表观遗传标记物5-羟甲基胞嘧啶（已显示区分良性痣和黑色素瘤的功能显著指标）的双标记。所有（18/18）转移性黑色素瘤病例显示MART-1阳性细胞中的5-羟甲基胞嘧啶核染色完全丧失，所有（10/10）结节痣病例显示MART-1阳性细胞中的5-羟甲基胞嘧啶核染色。此外，5-羟甲基胞嘧啶染色证实了转移性黑色素瘤在两个'模棱两可'的情况下，有利于诊断。因此，5-羟甲基胞嘧啶可能是一个有用的标记，以区分良性结节痣和转移性黑色素瘤的前哨淋巴结活检转移性黑色素瘤的评价。
Sentinel lymph node biopsies are conducted to stage patients with newly-diagnosed melanomas that have histopathologic attributes conferring defined levels of metastatic potential. Because benign nevic cells may also form ‘deposits’ in lymph nodes (nodal nevus), the pathologic evaluation for metastatic melanoma within sentinel lymph nodes can be challenging. 28 sentinel lymph node biopsy cases containing either metastatic melanoma (N=18) or nodal nevi (N=10) were retrieved from the archives of the Brigham and Women’s Hospital Department of Pathology (2011–2014). In addition, two sentinel lymph node cases that were favored to represent metastatic disease but whose histopathologic features were viewed as equivocal, with melanoma favored, were also included. Dual-labeling for the melanocyte lineage marker, MART-1, and the epigenetic marker 5-hydroxymethylcytosine, a functionally-significant indicator that has been shown to distinguish benign nevi from melanoma, was performed on all cases using immunohistochemistry and/or direct immunofluorescence. All (18 of 18) metastatic melanoma cases showed complete loss of 5-hydroxymethylcytosine nuclear staining in MART-1-positive cells and all (10 of 10) nodal nevus cases demonstrated 5-hydroxymethylcytosine nuclear staining in MART-1 positive cells. In addition, 5-hydroxymethylcytosine staining confirmed the favored diagnoses of metastatic melanoma in the two ‘equivocal’ cases. Thus, 5-hydroxymethylcytosine may be a useful adjunctive marker to distinguish between benign nodal nevi and metastatic melanoma during the evaluation of sentinel lymph node biopsies for metastatic melanoma.
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影响因子：
64.5
作者：
Wu H;Zhang Y
通讯作者：
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影响因子：
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作者：
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影响因子：
3.6
作者：
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影响因子：
1.1
作者：
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通讯作者：
Walsh, N
DOI：
10.6004/jnccn.2013.0055
发表时间：
2013-04-01
影响因子：
13.4
作者：
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通讯作者：
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