Modulation of growth, differentiation and glycoprotein synthesis by β‐ALL‐trans retinoic acid in a multicellular tumor spheroid model for squamous carcinoma of the head and neck
Modulation of growth, differentiation and glycoprotein synthesis by β‐ALL‐trans retinoic acid in a multicellular tumor spheroid model for squamous carcinoma of the head and neck
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β-ALL-反式视黄酸在头颈鳞状细胞癌多细胞肿瘤球模型中调节生长、分化和糖蛋白合成
DOI：
10.1002/ijc.2910440530
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发表时间：
1989
影响因子：
6.4
通讯作者：
R. Lotan
中科院分区：
文献类型：
--
作者：
P. Sacks;Victor Oke;B. Amos;T. Vasey;R. Lotan
从喉淋巴结转移瘤建立细胞系MDA 886 Ln。当作为多细胞肿瘤球体（MTS）生长时，它表现出鳞状分化。我们研究了全反式维甲酸（RA）对头颈部鳞状细胞癌MTS模型生长、分化和糖蛋白含量的影响。10 - 6至10 - 10 M RA以剂量依赖性方式抑制MTS的生长。生长抑制发生在RA处理（10−6 M）的3 - 5天之间。免疫组化和电泳分析显示，RA抑制鳞状分化（squames），外皮蛋白表达和角蛋白表达的形态学标志物。通过使用3 H-葡萄糖胺的代谢标记、使用125 I标记的小麦胚芽凝集素（WGA）原位标记聚丙烯酰胺凝胶、异硫代荧光素-WGA在冷冻切片中的定位以及唾液酸转移酶活性测定来检查糖蛋白表达。用10−6 M RA处理改变了糖蛋白的生物化学和形态学表达，WGA被证明优先与唾液酸残基结合。这种MTS模型对RA治疗的敏感性及其通过形态学、免疫组织化学和生物化学技术分析的能力表明，它将被证明有助于研究鳞状细胞癌中生长、分化和RA诱导的改变之间的关系。
Cell line MDA 886Ln was established from a laryngeal lymph node metastasis. When grown as a multicellular tumor spheroid (MTS), it exhibits squamous differentiation. We studied the effects of ß‐all‐trans retinoic acid (RA) on the growth, differentiation and glycoprotein content of this MTS model for squamous carcinomas of the head and neck. The growth of MTSs was inhibited in a dose‐dependent manner by 10−6 to 10−10 M RA. Growth inhibition occurred between 3 and 5 days of RA treatment (10−6 M). Immunohistochemical and electrophoretic analyses revealed that RA suppressed the morphological markers of squamous differentiation (squames), involucrin expression, and keratin expression. Glycoprotein expression was examined by metabolic labelling using 3H‐glucosamine, in situ labelling of polyacrylamide gels with 125l‐labelled wheat‐germ agglutinin (WGA), localization of fluorescein isothionate‐WGA in frozen sections, and determination of sialyltransferase activity. Treatment using 10−6 M RA altered glycoprotein expression both biochemically and morphologically, and WGA was shown to bind preferentially to sialic acid residues. The sensitivity of this MTS model to RA treatment and its ability to be analyzed through morphological, immunohistochemical and biochemical techniques suggest that it will prove useful in studying the relationships between growth, differentiation and RA‐induced alterations in squamous carcinomas.
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通过氢化可的松、乙酸视黄酯和生长抑制调节人角质形成细胞的外皮和包膜能力。
影响因子：
11.2
作者：
Cline,PR;Rice,RH
通讯作者：
Rice,RH
影响因子：
11.2
作者：
J. Rheinwald;M. Beckett
通讯作者：
J. Rheinwald;M. Beckett
全反式视黄酸体外调节人鳞状癌细胞的终末分化。
DOI：
--
发表时间：
1985
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Reiss,M;Pitman,SW;Sartorelli,AC
通讯作者：
Sartorelli,AC
类维生素A抑制培养的人表皮癌细胞中的转谷氨酰胺酶活性和包膜能力。
DOI：
10.1111/j.1432-0436.1985.tb00296.x
发表时间：
1985
期刊：
Differentiation; research in biological diversity
影响因子：
--
作者：
Thacher,SM;Coe,EL;Rice,RH
通讯作者：
Rice,RH
影响因子：
56.9
作者：
SUTHERLAND, RM
通讯作者：
SUTHERLAND, RM