Effects of Environmental and Water Quality Variables on Histamine-Producing Bacteria Concentration and Species in the Northern Gulf of Mexico.
Effects of Environmental and Water Quality Variables on Histamine-Producing Bacteria Concentration and Species in the Northern Gulf of Mexico.
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DOI：
10.1128/spectrum.04720-22
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发表时间：
2023-08-17
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
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作者：
鱼毒素（组胺）中毒是一种常见的海产品传播疾病，是由鱼组织分解过程中产生组胺的细菌（HPB）产生的毒素引起的。在实验室研究中，HPB和其他细菌种类的生长受到物理和化学性质的影响，但HPB的自然群落尚未得到很好的了解。为了确定原位环境和水质变量如何影响自然水生环境中HPB的密度，我们比较了2017年7月至2018年2月在墨西哥湾北部沿海潮汐河沿自然盐度梯度采集的水样中HPB的存在和丰度与环境温度、盐度、溶解氧、粪便大肠菌群、雄性特异性噬菌体、营养物质浓度、碳氮稳定同位素比率和C:N的关系。采用实时PCR，最可能数法定量水样中的HPB。通过16S rRNA基因序列鉴定HPB种。温度和盐度是影响HPB存在和浓度的主要因素。典型对应分析表明，不同的HPB与不同的环境条件有关。在温暖、高盐度的条件下发现了豆色光杆菌；planticola Raoultella是在较冷、较低盐度的条件下发现的；产气肠杆菌是在温暖、低盐度条件下发现的；莫氏摩根菌分布在大多数地点，与环境条件无关。这些结果表明，环境条件会影响天然存在的HPB丰度和种类组成，这可能表现为环境因素对组胺形成和鲭毒素中毒风险的不同影响。重要性：本研究确定了环境条件对墨西哥湾北部天然产生组胺的细菌的存在和丰度的影响。本研究表明，HPB丰度和物种组成与原位环境温度和盐度有关，其影响程度取决于特定的HPB物种。这一发现表明，捕鱼地点的环境条件可能影响人类因鲭鱼毒素（组胺）中毒而患病的风险。
Scombrotoxin (histamine) fish poisoning is a common seafood-borne illness attributed to toxin production by histamine-producing bacteria (HPB) in fish tissues during decomposition. In laboratory studies, growth of HPB and other bacterial species is affected by physical and chemical attributes, but natural communities of HPB are not well understood. To determine how in situ environmental and water quality variables may affect density of HPB in the natural aquatic environment, we compared presence and abundance of HPB to ambient temperature, salinity, dissolved oxygen, fecal coliforms, male-specific coliphage, nutrient concentrations, carbon and nitrogen stable isotope ratios, and C:N in water samples collected from July 2017 to February 2018 along a natural salinity gradient in a tidal river on the coast of northern Gulf of Mexico. HPB in water samples were quantified using a real-time PCR, most probable number method. HPB species were identified via 16S rRNA gene sequences. Temperature and salinity were determined to be the main factors driving HPB presence and concentration. Canonical correspondence analysis revealed that different HPB were associated with different environmental conditions. Photobacterium damselae was found under warmer, higher-salinity conditions; Raoultella planticola was found at colder, lower-salinity conditions; Enterobacter aerogenes was found at warmer, lower-salinity conditions; and Morganella morganii was found at most sites, independent of environmental conditions. These results showed that naturally occurring HPB abundance and species composition can be affected by environmental conditions, which could manifest in various potentials for histamine formation and scombrotoxin fish poisoning risk based on environmental factors. IMPORTANCE This study determined the effects of environmental conditions on presence and abundance of naturally occurring histamine-producing bacteria in the northern Gulf of Mexico. Here, we show that HPB abundance and species composition are related to in situ ambient temperature and salinity, with the magnitude of this effect dependent on the particular HPB species. This finding suggests that environmental conditions at fishing sites could affect the risk of human illness from scombrotoxin (histamine) fish poisoning.
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影响因子：
2.8
作者：
Henriques, Isabel S.;Alves, Artur;Correia, Antonio
通讯作者：
Correia, Antonio
DOI：
10.1038/nrmicro2259
发表时间：
2010-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
5.2
作者：
Jeffries TC;Schmitz Fontes ML;Harrison DP;Van-Dongen-Vogels V;Eyre BD;Ralph PJ;Seymour JR
通讯作者：
Seymour JR
影响因子：
2
作者：
Emborg, J;Dalgaard, P
通讯作者：
Dalgaard, P
影响因子：
5.2
作者：
Laurent, Arnaud;Fennel, Katja;Wanninkhof, Rik
通讯作者：
Wanninkhof, Rik