Effects of FGF-2 and IGF-1 on adult canine articular chondrocytes in type II collagen-glycosaminoglycan scaffolds in vitro.
Effects of FGF-2 and IGF-1 on adult canine articular chondrocytes in type II collagen-glycosaminoglycan scaffolds in vitro.
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FGF-2和IGF-1对体外II型胶原-糖胺聚糖支架中成年犬关节软骨细胞的影响。
DOI：
10.1016/j.joca.2004.12.013
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发表时间：
2005
影响因子：
7
通讯作者：
Spector,M
中科院分区：
文献类型：
--
作者：
Veilleux,N;Spector,M
目的：利用软骨细胞种子组织工程支架对软骨修复有一定的促进作用。本研究的目的是评估所选择的生长因子[成纤维细胞生长因子(FGF)-2和胰岛素样生长因子(IGF)-1]单独和联合对无血清（SF）培养基中生长的成年犬关节软骨细胞种子型II型胶原-糖胺聚糖（GAG）支架的影响。设计将约500,000个第二代软骨细胞植入4mm diameter×2mm厚的支架盘中。构建体在以下培养基中生长：含血清培养基；基础SF培养基；SF加5ng/ml FGF-2；SF添加25ng/ml FGF-2；SF加100ng/ml IGF-1；SF加5ng/ml FGF-2 + 100ng/ml IGF-1。第1天和第2周分别测定支架的DNA和GAG含量，第2周用放射性标记法测定支架的蛋白质和GAG合成率。同时进行组织学和II型胶原免疫组化。结果5ng/ml的fgf -2显著提高了细胞的生物合成活性和GAG的积累。组织学显示软骨细胞均匀分布在基质中，仅在2周后，该基质对GAG和II型胶原染色强烈。令人感兴趣的是胶原蛋白支架的快速降解，尽管支架是碳二亚胺交联的，以及结构的收缩。使用较高剂量的FGF-2和与IGF-1联合使用的效果不太明显。结论在添加5ng/ml FGF-2的SF培养基中培养的软骨细胞种子型II型胶原支架发生收缩，显示出放射性标记的硫酸盐掺入增加，并表现出软骨形成的定性迹象。
OBJECTIVEChondrocyte-seeded tissue engineering scaffolds hold the promise of enhancing certain cartilage repair procedures. The objective of this study was to evaluate the effects of selected growth factors [fibroblast growth factor (FGF)-2 and insulin-like growth factor (IGF)-1] individually and in combination on adult canine articular chondrocyte-seeded type II collagen–glycosaminoglycan (GAG) scaffolds grown in serum-free (SF) medium.DESIGNApproximately 500,000 second passage chondrocytes were seeded into discs of the scaffold, 4mm diameter×2mm thick. The constructs were grown in the following media: serum-containing medium; a basal SF medium; SF with 5ng/ml FGF-2; SF with 25ng/ml FGF-2; SF with 100ng/ml IGF-1; and SF with 5ng/ml FGF-2 plus 100ng/ml IGF-1. The DNA and GAG contents of the scaffolds were determined after 1 day and 2 weeks and the protein and GAG synthesis rates determined at 2 weeks using radiolabels. Histology and type II collagen immunohistochemistry were also performed.RESULTSFGF-2 at 5ng/ml was found to substantially increase the biosynthetic activity of the cells and the accumulation of GAG. The histology demonstrated chondrocytes uniformly distributed through a matrix that stained intensely for GAG and type II collagen after only 2 weeks. Of interest were the rapid degradation of the collagen scaffold, despite the fact that the scaffold was carbodiimide cross-linked, and the contraction of the constructs. There were less pronounced effects using the higher dose of FGF-2 and the combination with IGF-1.CONCLUSIONSChondrocyte-seeded type II collagen scaffolds cultured in SF medium supplemented with 5ng/ml FGF-2 undergo contraction, demonstrate an increase in construct incorporation of radiolabeled sulfate, and display qualitative signs of chondrogenesis.
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用于椎间盘组织生长的胶原蛋白-GAG 基质
DOI：
--
发表时间：
2002
期刊：
影响因子：
--
作者：
D. Hastreiter
通讯作者：
D. Hastreiter
II 型胶原蛋白对软骨细胞对 TGF-β1 调节反应的影响。
DOI：
10.1006/excr.1998.4032
发表时间：
1998
期刊：
Experimental cell research.
影响因子：
--
作者：
Qi,WN;Scully,SP
通讯作者：
Scully,SP
影响因子：
2.8
作者：
Kinner, B;Spector, M
通讯作者：
Spector, M
体内软骨由生长因子调节的关节软骨细胞形成。
影响因子：
4.2
作者：
D. Bradham;W. Horton
通讯作者：
W. Horton
影响因子：
2.9
作者：
KIM, YJ;SAH, RLY;GRODZINSKY, AJ
通讯作者：
GRODZINSKY, AJ