A collagen-GAG matrix for the growth of intervertebral disc tissue
A collagen-GAG matrix for the growth of intervertebral disc tissue
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用于椎间盘组织生长的胶原蛋白-GAG 基质
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
D. Hastreiter
中科院分区：
文献类型：
--
作者：
D. Hastreiter
椎间盘（IVD）退变和突出是一个重要的问题，在航空领域更是如此。IVD在太空飞行中也会发生变化。目前对IVD问题的治疗可能会产生不利的长期后果。本论文研究了一种多孔的、可生物降解的胶原-糖胺聚糖（CG）基质在体外不同培养环境中用于IVD纤维环（AF）组织的生长，并在体内实验中作为AF缺损的植入物。第一部分涉及其他研究中使用的CG基质的生产和表征。此外，I型、II型和50/50 I/I型CG基质具有几乎相等的孔径、糖胺聚糖含量和溶胀比。其次，通过在基质之上或之间培养AF外植体来评估椎间盘细胞生长到基质中的能力。细胞能够从外植体迁移至1 cm，这意味着该尺寸的AF缺损可以用CG基质填充。在第三个实验中，外植体和基质构建体在设计用于模拟微重力的隔壁生物反应器中培养。静态培养作为对照。生物反应器外植体含水量更高，细胞增殖更快。该实验可作为AF外植体空间飞行实验的地面对照。第四个研究部分研究了胶原成分对接种到CG基质中的AF细胞的增殖和生物合成反应的影响。通过使用具有上述匹配特征的I型、II型和I/II型CG基质改变胶原蛋白含量。尽管结果表明II型基质的性能略好，但在基质类型之间未观察到重大差异。第五项研究是一项犬体内研究，旨在评估CG基质结构在人工创建的缺损中辅助AF组织再生的能力。未处理与植入未接种和接种细胞的CG基质进行比较。在基质植入的缺损中观察到更多的组织生长和更一致的细胞过多。该研究表明，基质具有改善IVD中伤口愈合的潜力。论文导师：Myron Spector博士职务：骨科教授（生物材料）
Intervertebral disc (IVD) degeneration and herniation is a significant problem, more so in the aviation field. The IVD also changes during spaceflight. Current treatments for IVD problems can have unfavorable long-term consequences. This thesis investigated a porous, biodegradable collagen-glycosaminoglycan (CG) matrix for the growth of IVD annulus fibrosus (AF) tissue in vitro in varied culture environments and in an in vivo experiment as an implant for defects in the AF. Five experiments were performed. The first component involved the manufacture and characterization of the CG matrices used in the other studies. Additionally, type I, type II, and 50/50 type I/I CG matrices were made with nearly equal pore diameters, glycosaminoglycan content, and swelling ratios. Second, the capability of intervertebral disc cells to grow into the matrices was assessed by culturing AF explants on top of or between matrices. Cells were able to migrate up to 1 cm from the explant, implying that AF defects of this size could be filled with CG matrices. In the third experiment, explants and explant-matrix constructs were cultured in a rotating-wall bioreactor designed to simulate microgravity. Static culture served as a control. Bioreactor explants were more hydrated and had greater cellular proliferation. This experiment could serve as the ground control for a spaceflight experiment of AF explants. The fourth research component studied the effect of collagen composition on the proliferative and biosynthetic responses of AF cells seeded into CG matrices. Collagen content was varied by using the type I, type II, and type I/II CG matrices with matched characteristics mentioned above. Although the results indicated that the type II matrix performed slightly better, no major differences were seen among the matrix types. The fifth investigation was a canine in vivo study to assess the capability of the CG matrix constructs to aid in regeneration of AF tissue in surgically-created defects. No treatment was compared with implantation of unseeded and cellseeded CG matrices. More tissue grew and more consistent hypercellularity was observed in defects with matrix implantation. From this research it has been shown that the matrix has potential for improving wound healing in the IVD. Thesis Supervisor: Myron Spector, Ph.D. Title: Professor of Orthopedic Surgery (Biomaterials)
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DOI：
10.1002/jbm.820291108
发表时间：
1995-11
期刊：
Journal of biomedical materials research
影响因子：
--
作者：
K. Weadock;E. J. Miller;Lisa D. Bellincampi;J. Zawadsky;M. Dunn
通讯作者：
K. Weadock;E. J. Miller;Lisa D. Bellincampi;J. Zawadsky;M. Dunn
DOI：
10.1002/jbm.820290915
发表时间：
1995-09-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
CHU, CR;COUTTS, RD;AMIEL, D
通讯作者：
AMIEL, D
影响因子：
--
作者：
Rong, Y;Sugumaran, G;Spector, M
通讯作者：
Spector, M
影响因子：
2.9
作者：
KIM, YJ;SAH, RLY;GRODZINSKY, AJ
通讯作者：
GRODZINSKY, AJ
影响因子：
2
作者：
T. Oegema
通讯作者：
T. Oegema