Self-collected mid-turbinate swabs for the detection of respiratory viruses in adults with acute respiratory illnesses.
Self-collected mid-turbinate swabs for the detection of respiratory viruses in adults with acute respiratory illnesses.
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DOI：
10.1371/journal.pone.0021335
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
McGeer AJ
中科院分区：
文献类型：
--
作者：
Larios OE;Coleman BL;Drews SJ;Mazzulli T;Borgundvaag B;Green K;STOP-Flu Study Group;McGeer AJ
呼吸道病毒检测的金标准是由卫生保健工作者收集的鼻咽拭子。中鼻甲拭子是一种替代方法，因为它们易于收集，并且可能由患者自行收集。本研究的目的是比较蜂群MT拭子与蜂群NP拭子的呼吸道病毒分离情况。从2008年10月开始，18至69岁的健康成年人被招募到一个队列中，并对流感症状进行了随访。他们被要求在发烧或急性发作时出现两种或两种以上呼吸道症状后尽快采集NP和MT拭子。使用Seeplex®RV12多重PCR检测试剂盒检测拭子是否感染呼吸道病毒。从38名有症状的受试者中同时采集76对NP和MT拭子。其中29对（38%）通过NP或MT拭子或两者均呈阳性。69例（91%）结果一致。2份（3%）hCV OC43/HKU1和1份（1%）鼻病毒A/B经NP检测呈阳性，但MT检测呈阴性。hCV 229E/NL63、hCV OC43/HKU1、呼吸道合胞病毒A和流感B各1份经MT检测呈阳性，但NP检测呈阴性。蜂群MT拭子对多种呼吸道病毒诊断敏感。鉴于MT采集的便便性和两种拭子之间相似的结果，MT拭子可能应该是门诊研究中首选的呼吸细胞采集方法。鉴于这些数据，应该进行更大规模的研究，以确保这仍然是正确的，并且还应该收集患者对收集方法的偏好的数据。
The gold standard for respiratory virus testing is a nasopharyngeal (NP) swab, which is collected by a healthcare worker. Midturbinate (MT) swabs are an alternative due to their ease of collection and possible self-collection by patients. The objective of this study was to compare the respiratory virus isolation of flocked MT swabs compared to flocked NP swabs. Beginning in October 2008, healthy adults aged 18 to 69 years were recruited into a cohort and followed up for symptoms of influenza. They were asked to have NP and MT swabs taken as soon as possible after the onset of a fever or two or more respiratory symptoms with an acute onset. The swabs were tested for viral respiratory infections using Seeplex® RV12 multiplex PCR detection kit. Seventy six pairs of simultaneous NP and MT swabs were collected from 38 symptomatic subjects. Twenty nine (38%) of these pairs were positive by either NP or MT swabs or both. Sixty nine (91%) of the pair results were concordant. Two samples (3%) for hCV OC43/HKU1 and 1 sample (1%) for rhinovirus A/B were positive by NP but negative by MT. One sample each for hCV 229E/NL63, hCV OC43/HKU1, respiratory syncytial virus A, and influenza B were positive by MT but negative by NP. Flocked MT swabs are sensitive for the diagnosis of multiple respiratory viruses. Given the ease of MT collection and similar results between the two swabs, it is likely that MT swabs should be the preferred method of respiratory cell collection for outpatient studies. In light of this data, larger studies should be performed to ensure that this still holds true and data should also be collected on the patient preference of collection methods.
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DOI：
10.1016/s1386-6532(07)70004-5
发表时间：
2007-11
期刊：
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
影响因子：
--
作者：
Ginocchio CC
通讯作者：
Ginocchio CC
影响因子：
9.4
作者：
Abu-Diab, Afaf;Azzeh, Maysa;Hindiyeh, Musa Y.
通讯作者：
Hindiyeh, Musa Y.
DOI：
10.1164/rccm.201002-0221oc
发表时间：
2010-11-15
影响因子：
24.7
作者：
DeVincenzo, John P.;Wilkinson, Tom;Lambkin-Williams, Robert
通讯作者：
Lambkin-Williams, Robert
影响因子：
12.7
作者：
Kaiser, L;Fritz, RS;Hayden, FG
通讯作者：
Hayden, FG
影响因子：
12.7
作者：
To, Kelvin K. W.;Chan, Kwok-Hung;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung