Genome sequence of an Australian kangaroo, Macropus eugenii, provides insight into the evolution of mammalian reproduction and development.
Genome sequence of an Australian kangaroo, Macropus eugenii, provides insight into the evolution of mammalian reproduction and development.
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DOI：
10.1186/gb-2011-12-8-r81
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发表时间：
2011-08-29
期刊：
影响因子：
12.3
通讯作者：
Worley KC
中科院分区：
文献类型：
--
作者：
Renfree MB;Papenfuss AT;Deakin JE;Lindsay J;Heider T;Belov K;Rens W;Waters PD;Pharo EA;Shaw G;Wong ES;Lefèvre CM;Nicholas KR;Kuroki Y;Wakefield MJ;Zenger KR;Wang C;Ferguson-Smith M;Nicholas FW;Hickford D;Yu H;Short KR;Siddle HV;Frankenberg SR;Chew KY;Menzies BR;Stringer JM;Suzuki S;Hore TA;Delbridge ML;Patel HR;Mohammadi A;Schneider NY;Hu Y;O'Hara W;Al Nadaf S;Wu C;Feng ZP;Cocks BG;Wang J;Flicek P;Searle SM;Fairley S;Beal K;Herrero J;Carone DM;Suzuki Y;Sugano S;Toyoda A;Sakaki Y;Kondo S;Nishida Y;Tatsumoto S;Mandiou I;Hsu A;McColl KA;Lansdell B;Weinstock G;Kuczek E;McGrath A;Wilson P;Men A;Hazar-Rethinam M;Hall A;Davis J;Wood D;Williams S;Sundaravadanam Y;Muzny DM;Jhangiani SN;Lewis LR;Morgan MB;Okwuonu GO;Ruiz SJ;Santibanez J;Nazareth L;Cree A;Fowler G;Kovar CL;Dinh HH;Joshi V;Jing C;Lara F;Thornton R;Chen L;Deng J;Liu Y;Shen JY;Song XZ;Edson J;Troon C;Thomas D;Stephens A;Yapa L;Levchenko T;Gibbs RA;Cooper DW;Speed TP;Fujiyama A;Graves JA;O'Neill RJ;Pask AJ;Forrest SM;Worley KC
我们目前的基因组序列的tammar袋鼠，Macropus eugenii，这是袋鼠家族的成员和标志性的跳跃哺乳动物，象征着澳大利亚的第一个代表进行测序。tammar有许多不寻常的生物学特征，包括任何哺乳动物中最长的胚胎滞育期，极其同步的季节性繁殖和在一个明确的育儿袋内长期而复杂的哺乳。像其他有袋类动物一样，它会生育高度成熟的幼仔，并有少量非常大的染色体，使其成为基因组学，生殖和发育的宝贵模型。已使用桑格测序法对基因组进行了2倍覆盖率的测序，并通过额外的下一代测序和整合广泛的物理和连锁图谱进行了增强，以构建基因组组装。我们还对许多组织和发育时间点的tammar转录组进行了测序。我们对这些数据的分析揭示了哺乳动物的生殖，发育和基因组进化：生殖和哺乳基因的创新，生殖细胞基因的快速进化，以及不完全的，位点特异性的X失活。我们还观察到新的反转录转座子和高度重排的主要组织相容性复合体，许多I类基因位于复合体外。tammar HOX簇中的新型microRNA揭示了新的潜在哺乳动物HOX调控元件。对这些资源的分析增强了我们对有袋动物基因进化的理解，确定了有袋动物特有的保守非编码元件和跨一系列生物系统的关键基因，包括生殖，发育和免疫，并为有袋动物和哺乳动物生物学和基因组进化提供了新的见解。
We present the genome sequence of the tammar wallaby, Macropus eugenii, which is a member of the kangaroo family and the first representative of the iconic hopping mammals that symbolize Australia to be sequenced. The tammar has many unusual biological characteristics, including the longest period of embryonic diapause of any mammal, extremely synchronized seasonal breeding and prolonged and sophisticated lactation within a well-defined pouch. Like other marsupials, it gives birth to highly altricial young, and has a small number of very large chromosomes, making it a valuable model for genomics, reproduction and development. The genome has been sequenced to 2 × coverage using Sanger sequencing, enhanced with additional next generation sequencing and the integration of extensive physical and linkage maps to build the genome assembly. We also sequenced the tammar transcriptome across many tissues and developmental time points. Our analyses of these data shed light on mammalian reproduction, development and genome evolution: there is innovation in reproductive and lactational genes, rapid evolution of germ cell genes, and incomplete, locus-specific X inactivation. We also observe novel retrotransposons and a highly rearranged major histocompatibility complex, with many class I genes located outside the complex. Novel microRNAs in the tammar HOX clusters uncover new potential mammalian HOX regulatory elements. Analyses of these resources enhance our understanding of marsupial gene evolution, identify marsupial-specific conserved non-coding elements and critical genes across a range of biological systems, including reproduction, development and immunity, and provide new insight into marsupial and mammalian biology and genome evolution.
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影响因子：
12.3
作者：
Al Nadaf, Shafagh;Waters, Paul D.;Graves, Jennifer A. M.
通讯作者：
Graves, Jennifer A. M.
DOI：
10.1002/cyto.990120613
发表时间：
1991-01-01
期刊：
CYTOMETRY
影响因子：
--
作者：
BOSCHMAN, GA;RENS, W;ATEN, JA
通讯作者：
ATEN, JA
影响因子：
1.6
作者：
Carone, Dawn M.;Longo, Mark S.;O'Neill, Rachel J.
通讯作者：
O'Neill, Rachel J.
影响因子：
5.8
作者：
Chau, YM;Pando, S;Taylor, HS
通讯作者：
Taylor, HS
影响因子：
2.3
作者：
Chung, J. W.;Pask, A. J.;Renfree, M. B.
通讯作者：
Renfree, M. B.