Enhanced UV-mediated free radical generation; DNA and mitochondrial damage caused by retinol supplementation
Enhanced UV-mediated free radical generation; DNA and mitochondrial damage caused by retinol supplementation
复制标题
增强紫外线介导的自由基产生；
DOI：
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发表时间：
2003
影响因子：
3.1
通讯作者：
J. Moreira
中科院分区：
文献类型：
--
作者：
F. Klamt;F. Dal;E. A. Bernard;J. Moreira
类维生素a补充剂已被用于治疗各种人类疾病。我们和其他人已经证明，在体外和体内，视黄醇治疗会导致自由基的产生，增加铁的摄取、铁的储存和氧化损伤。在这里，我们研究了视黄醇在原代培养的哺乳动物细胞中对紫外线介导的自由基生成、生物分子氧化损伤和细胞活力下降可能的协同作用。视黄醇处理（7 μM）导致紫外线介导的自由基生成增加三倍，脂质过氧化增加40%。与只用视黄醇输送溶剂（0.1%乙醇）处理的对照细胞相比，添加视黄醇的紫外线照射培养细胞的DNA断裂和线粒体氧化损伤也显著增加。当铁螯合剂1,10-菲罗啉（100 μM）或OH^•清除剂甘露醇（1 mM）共同给药时，所有测量值恢复到控制值。而不是防止自由基的产生，视黄醇似乎加强了紫外线介导的氧化损伤，降低了培养细胞的细胞活力。我们认为，视黄醇增强铁的摄取和储存以及芬顿反应产生的活性氧的增加可能与紫外线照射协同作用，导致细胞氧化损伤。
Retinoid supplementation has been therapeutically used against various human disorders. We and others have demonstrated that retinol treatment causes free radical generation and increased iron uptake, iron storage and oxidative damage, both in vitro and in vivo . Here, we investigate the possible synergistic effect of retinol on UV-mediated free radical generation, oxidative damage to biomolecules and decreased cellular viability in primary cultured mammalian cells. Retinol treatment (7 μM) resulted in a threefold increase in UV-mediated free radical generation and a 40% increase in lipoperoxidation. DNA fragmentation and mitochondrial oxidative damage also increased significantly in retinol-supplemented UV-irradiated cultured cells as compared to UV-irradiated control cells, which were only treated with the solvent used to deliver the retinol (0.1% ethanol). All measurements were restored to control values when an iron chelator, 1,10-phenanthroline (100 μM), or an OH^• scavenger, mannitol (1 mM), was co-administrated. Rather than protecting against free radical generation, retinol seems to enhance UV-mediated oxidative damage and decreases cellular viability in cultured cells. We suggest that retinol-enhanced iron uptake and storage and increased reactive oxygen species generated by the Fenton reaction may act synergistically with UV-irradiation in causing oxidative damage to cells.
影响因子：
7.4
作者：
Zhang, XS;Rosenstein, BS;Wei, HC
通讯作者：
Wei, HC
DOI：
10.1007/bf00054305
发表时间：
1991
期刊：
Cancer causes & control : CCC
影响因子：
--
作者：
Mayne,ST;Graham,S;Zheng,TZ
通讯作者：
Zheng,TZ