Complete mitochondrial genomes of Taenia multiceps, T. hydatigena and T. pisiformis: additional molecular markers for a tapeworm genus of human and animal health significance.
Complete mitochondrial genomes of Taenia multiceps, T. hydatigena and T. pisiformis: additional molecular markers for a tapeworm genus of human and animal health significance.
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DOI：
10.1186/1471-2164-11-447
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发表时间：
2010-07-22
期刊：
影响因子：
4.4
通讯作者：
Cai XP
中科院分区：
文献类型：
--
作者：
Jia WZ;Yan HB;Guo AJ;Zhu XQ;Wang YC;Shi WG;Chen HT;Zhan F;Zhang SH;Fu BQ;Littlewood DT;Cai XP
线粒体基因组提供了丰富的分子变异来源，在分子生态学、群体遗传学和进化生物学中被证明和广泛应用。带绦虫属包括多种对人类和兽医具有重要意义的带绦虫寄生虫。在这里，我们添加T的mt基因组的完整序列。multiceps，T. Hydatigena和T.豌豆形虫属4个已发表的mtDNA数据集进行比较。七个完整的MT带绦虫属物种的基因组被用来比较和对比基因组内和基因组之间的变化，估计属的遗传，并开发新的分子标记作为扩展的线粒体工具包的一部分。T. multiceps，T. Hydatigena和T. pisiformis基因的大小分别为13，693、13，492和13，387 bp，构成了扁形虫基因的常见互补序列。蛋白质编码基因的起始密码子和终止密码子包括在其他扁形动物mt基因组中常见的密码子，但在T.豌豆形肌线粒体DNA的系统发育分析提供了新的估计带绦虫的相互关系。滑动窗口分析显示nad6，nad5，atp6，nad3和nad2是单位长度上变异最多的基因，其中nad5的核苷酸多样性最高。能够扩增nad1，rrnS和nad5基因中可变DNA片段的新引物对被设计在计算机上，并被测试为现有带绦虫线粒体标记的可能替代品。随着完整的mtDNA的可用性7带绦虫属物种，我们已经表明，氨基酸分析提供了一个强大的估计，该属，明显不同于形态学估计或使用部分基因，理解重要的带绦虫的进化辐射的影响。完整的带绦虫mtDNA的核苷酸比对和滑动窗口分析表明，许多替代基因区域可能捕获更大的核苷酸变异比目前追求的分子标记。新的PCR引物开发的比较有丝分裂基因组分析的带绦虫物种，扩展使用线粒体标记的分子生态学，群体遗传学和诊断。
Mitochondrial genomes provide a rich source of molecular variation of proven and widespread utility in molecular ecology, population genetics and evolutionary biology. The tapeworm genus Taenia includes a diversity of tapeworm parasites of significant human and veterinary importance. Here we add complete sequences of the mt genomes of T. multiceps, T. hydatigena and T. pisiformis, to a data set of 4 published mtDNAs in the same genus. Seven complete mt genomes of Taenia species are used to compare and contrast variation within and between genomes in the genus, to estimate a phylogeny for the genus, and to develop novel molecular markers as part of an extended mitochondrial toolkit. The complete circular mtDNAs of T. multiceps, T. hydatigena and T. pisiformis were 13,693, 13,492 and 13,387 bp in size respectively, comprising the usual complement of flatworm genes. Start and stop codons of protein coding genes included those found commonly amongst other platyhelminth mt genomes, but the much rarer initiation codon GTT was inferred for the gene atp6 in T. pisiformis. Phylogenetic analysis of mtDNAs offered novel estimates of the interrelationships of Taenia. Sliding window analyses showed nad6, nad5, atp6, nad3 and nad2 are amongst the most variable of genes per unit length, with the highest peaks in nucleotide diversity found in nad5. New primer pairs capable of amplifying fragments of variable DNA in nad1, rrnS and nad5 genes were designed in silico and tested as possible alternatives to existing mitochondrial markers for Taenia. With the availability of complete mtDNAs of 7 Taenia species, we have shown that analysis of amino acids provides a robust estimate of phylogeny for the genus that differs markedly from morphological estimates or those using partial genes; with implications for understanding the evolutionary radiation of important Taenia. Full alignment of the nucleotides of Taenia mtDNAs and sliding window analysis suggests numerous alternative gene regions are likely to capture greater nucleotide variation than those currently pursued as molecular markers. New PCR primers developed from a comparative mitogenomic analysis of Taenia species, extend the use of mitochondrial markers for molecular ecology, population genetics and diagnostics.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
4
作者：
CABRERA, PA;HARAN, G;CARBALLO, M
通讯作者：
CARBALLO, M
DOI：
10.1016/j.diagmicrobio.2004.03.013
发表时间：
2004-07-01
影响因子：
2.9
作者：
González, LM;Montero, E;Gárate, T
通讯作者：
Gárate, T
影响因子：
1.3
作者：
de Queiroz, A;Alkire, NL
通讯作者：
Alkire, NL
PRIFI：使用相关序列的多重比对来找到用于放大同源物的引物。
影响因子：
14.9
作者：
Fredslund, J;Schauser, L;Madsen, LH;Sandal, N;Stougaard, J
通讯作者：
Stougaard, J