Autoantibodies against the envelope proteins of endogenous retroviruses K102 and K108 in patients with systemic lupus erythematosus correlate with active disease.
Autoantibodies against the envelope proteins of endogenous retroviruses K102 and K108 in patients with systemic lupus erythematosus correlate with active disease.
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系统性红斑狼疮患者体内抗内源性逆转录病毒K102和K108包膜蛋白的自身抗体与活动性疾病相关
DOI：
10.55563/clinexprheumatol/2kg1d8
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发表时间：
2022-07
影响因子：
3.7
通讯作者：
Mustelin T
中科院分区：
文献类型：
--
作者：
Khadjinova AI;Wang X;Laine A;Ukadike K;Eckert M;Stevens A;Bengtsson AA;Lood C;Mustelin T
为了确定系统性红斑狼疮(SLE)患者是否表达能够产生包膜(Env)蛋白的人类内源性逆转录病毒K(Herv-K)前病毒，以及相关的针对Env蛋白的自身抗体，SLE是一种以I型干扰素升高为特征的疾病，使人联想到抗病毒免疫。用重组Env蛋白和来自SLE活动期(n=60)或非活动期(n=49)、健康对照组(n=47)、其他风湿性疾病(n=59)以及狼疮活动期(n=30)或非活动期(n=30)的儿童和17名健康儿童的血清进行ELISA检测。抗体反应性评估与患者的临床和实验室参数的相关性。用RNA-Seq方法分析HERV-K转录本在SLE白细胞中的表达。成人和儿童SLE患者的抗Herv-K Env自身抗体滴度均高于健康对照组或干燥综合征、大小血管炎或银屑病关节炎患者。在10个SLE患者表达的基因座中，仅有两个HERV-K基因座可产生Env，即HERV-K102和-K108。我们的数据显示，HERV-K前病毒在SLE中表达，并且HERV-K编码的Env蛋白在患者中引起免疫反应，特别是在活动期。
To determine if patients with systemic lupus erythematosus (SLE), a disease characterised by elevated type I interferons reminiscent of anti-viral immunity, have expression of human endogenous retrovirus K (HERV-K) proviruses capable of producing envelope (Env) protein, as well as associated autoantibodies against the Env protein. ELISAs were conducted with recombinant Env protein and sera from SLE patients with active (n=60) or inactive (n=49) disease, healthy controls (n=47), other rheumatic disorders (n=59), as well as plasma from paediatric lupus patients with active (n=30) or inactive (n=30) disease, and 17 healthy children. Antibody reactivity was evaluated for correlations with clinical and laboratory parameters of the patients. Expression of HERV-K transcripts were profiled in SLE leukocytes by RNA-Seq. Both adult and paediatric SLE patients had autoantibodies against HERV-K Env with higher titers than healthy controls or patients with Sjögren’s syndrome, small- or large-vessel vasculitis, or psoriatic arthritis. Transcripts from only two HERV-K loci capable of producing Env, HERV-K102 and -K108, were detected among the 10 expressed loci in SLE patients. Our data reveal that HERV-K proviruses are expressed in SLE and that the HERV-K-encoded Env protein elicits an immune response in patients, particularly during active disease.
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影响因子：
4.4
作者：
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影响因子：
3.3
作者：
de Mulder M;SenGupta D;Deeks SG;Martin JN;Pilcher CD;Hecht FM;Sacha JB;Nixon DF;Michaud HA
通讯作者：
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影响因子：
5.4
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通讯作者：
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影响因子：
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作者：
BLOMBERG, J;NIVED, O;STURFELT, G
通讯作者：
STURFELT, G
影响因子：
7.3
作者：
Mustelin T;Ukadike KC
通讯作者：
Ukadike KC