Dissection of genetic mechanisms governing the expression of serum retroviral gp70 implicated in murine lupus nephritis.
Dissection of genetic mechanisms governing the expression of serum retroviral gp70 implicated in murine lupus nephritis.
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遗传机制的解剖，涉及与鼠狼疮炎有关的血清逆转录病毒GP70的表达。
DOI：
10.4049/jimmunol.181.4.2846
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发表时间：
2008-08-15
影响因子：
4.4
通讯作者：
Izui, Shozo
中科院分区：
文献类型：
--
作者：
Baudino, Lucie;Yoshinobu, Kumiko;Morito, Naoki;Kikuchi, Shuichi;Fossati-Jimack, Liliane;Morley, Bernard J.;Vyse, Timothy J.;Hirose, Sachiko;Jorgensen, Trine N.;Tucker, Rebecca M.;Roark, Christina L.;Kotzin, Brian L.;Evans, Leonard H.;Izui, Shozo
内源性逆转录病毒包膜糖蛋白gp 70与鼠狼疮性肾炎有关，由肝细胞分泌为急性期蛋白，并被认为是内源性异嗜性病毒NZB-X1的产物。然而，由于内源性多变性（PT）和修饰的多变性（mPT）病毒编码与异嗜性gp 70密切相关的gp 70，因此这些病毒可能是血清gp 70的额外来源。为了更好地了解血清gp 70表达的遗传基础，我们分析了异嗜性，PT或mPT gp 70 RNA在肝脏中的丰度和相应的前病毒在不同品系的小鼠，包括两个不同的Sgp（血清gp 70生产）同源小鼠的基因组组成。我们的研究结果表明，不同的病毒gp 70 RNA的表达是显着的异质性在不同的小鼠品系和血清gp 70的生产水平是由多个结构和调控基因调节。此外，PT和mPT gp 70对血清gp 70的显著贡献通过在129只小鼠中检测PT和mPT而非异嗜性转录物而揭示，并且通过在Sgp 3同源小鼠中血清gp 70水平与PT和mPT gp 70 RNA的丰度比与异嗜性gp 70 RNA的丰度更密切的相关性而揭示。此外，注射脂多糖选择性上调异嗜性和mPT gp 70 RNA的表达，但不PT gp 70 RNA。我们的数据表明，血清gp 70的遗传起源比以前认为的更异质，不同的逆转录病毒gp 70在生理与炎症条件下的差异调节。
The endogenous retroviral envelope glycoprotein, gp70, implicated in murine lupus nephritis is secreted by hepatocytes as an acute phase protein, and has been believed to be a product of an endogenous xenotropic virus, NZB-X1. However, since endogenous polytropic (PT) and modified polytropic (mPT) viruses encode gp70s that are closely related to xenotropic gp70, these viruses can be additional sources of serum gp70. To better understand the genetic basis of the expression of serum gp70, we analyzed the abundance of xenotropic, PT or mPT gp70 RNAs in livers and the genomic composition of corresponding proviruses in various strains of mice, including two different Sgp (serum gp70 production) congenic mice. Our results demonstrated that the expression of different viral gp70 RNAs was remarkable heterogeneous among various mouse strains and that the level of serum gp70 production was regulated by multiple structural and regulatory genes. In addition, a significant contribution of PT and mPT gp70s to serum gp70 was revealed by the detection of PT and mPT, but not xenotropic transcripts in 129 mice and by a closer correlation of serum levels of gp70 with the abundance of PT and mPT gp70 RNAs than with that of xenotropic gp70 RNA in Sgp3 congenic mice. Furthermore, the injection of lipopolysaccharides selectively up-regulated the expression of xenotropic and mPT gp70 RNAs, but not PT gp70 RNA. Our data indicate that the genetic origin of serum gp70 is more heterogeneous than previously believed, and that distinct retroviral gp70s are differentially regulated in physiological vs. inflammatory conditions.
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影响因子：
64.8
作者：
ELDER, JH;JENSEN, FC;LERNER, RA
通讯作者：
LERNER, RA
影响因子：
5.4
作者：
FRANKEL, WN;STOYE, JP;COFFIN, JM
通讯作者：
COFFIN, JM
影响因子：
15.3
作者：
IZUI, S;KELLEY, VE;DIXON, FJ
通讯作者：
DIXON, FJ
影响因子：
5.4
作者：
MERINO, R;FOSSATI, L;IZUI, S
通讯作者：
IZUI, S
DOI：
10.1084/jem.149.5.1099
发表时间：
1979-05-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Izui S;McConahey PJ;Theofilopoulos AN;Dixon FJ
通讯作者：
Dixon FJ