Buffer Standards for pH Measurement of N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic Acid (HEPES) for I = 0.16 mol.kg from 5 to 55 degrees C.
Buffer Standards for pH Measurement of N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic Acid (HEPES) for I = 0.16 mol.kg from 5 to 55 degrees C.
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DOI：
10.1007/s10953-009-9378-3
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发表时间：
2009-04-01
影响因子：
1.2
通讯作者：
Cline, Kevin L.
中科院分区：
文献类型：
--
作者：
Roy, Rabindra N.;Roy, Lakshmi N.;Ashkenazi, Shahaf;Wollen, Joshua T.;Dunseth, Craig D.;Fuge, Michael S.;Durden, Jared L.;Roy, Chandra N.;Hughes, Hannah M.;Morris, Brett T.;Cline, Kevin L.
本文报道了N-(2-羟乙基)哌嗪-N‘-2-乙磺酸(HEPES)在5~55°C(包括37℃)12℃下的第二离解常数pK2。本文报道了8种等渗盐水缓冲溶液(I=0.16mol.kg−1)的pK2值，其中包括：(A)HEPES(0.01mol.kg−1)+NaHEPES(0.01mol.kg−1)+NaCl0.15mol.kg−1；(B)HEPES(0.02mol.kg−1)+NaHEPES(0.02mol.kg−1)+氯化钠(0.14mol.kg−1)；(C)HEPES(0.03mol.kg−1)+NaHEPES(0.03mol.kg−1)+氯化钠(0.13mol.kg−1)；(D)HEPES(0.04mol.kg−1)+NaHEPES(0.04mol.kg−1)+−(0.12mol.(E)HEPES(0.05mol.kg−1)+NaHEPES(0.05mol.kg−1)+氯化钠(0.11mol.kg−1)；(F)HEPES(0.06mol.kg−1)+NaHEPES(0.06mol.kg−1)+氯化钠(0.10mol.kg−1)；(G)HEPES(0.07mol.kg−1)+NaHEPES(0.07mol.kg−1)+−(0.09mol.(H)HEPES(0.08mol.kg−-1)+NaHEPES(0.08mol.kg−-1)+氯化钠(0.08mol.kg−-1)。计算了从5°C到55°C的所有八种缓冲溶液的常规PAH值。根据NBS/NIST标准，在生理磷酸盐标准和四种缓冲溶液之间确定了在25℃和37℃下进行液结校正后的操作pH值。当i=0.16mol.kg−1时，建议将其作为生理液体的pH标准，范围为PH7.3至7.5.
The values of the second dissociation constant, pK2 of N-(2-hydroxyethyl) piperazine-N’-2-ethanesulfonic acid (HEPES) have been reported at 12 temperatures over the temperature range 5 to 55°C, including 37°C. This paper reports the results for the paH of eight isotonic saline buffer solutions with an I = 0.16 mol•kg−1 including compositions: (a) HEPES (0.01 mol•kg−1) + NaHEPES (0.01 mol•kg−1) + NaCl (0.15 mol•kg−1); (b) HEPES (0.02 mol•kg−1) + NaHEPES (0.02 mol•kg−1) + NaCl (0.14 mol•kg−1); (c) HEPES (0.03 mol•kg−1) + NaHEPES (0.03 mol•kg−1) + NaCl (0.13 mol•kg−1); (d) HEPES (0.04 mol•kg−1) + NaHEPES (0.04 mol•kg−1) + NaCl (0.12 mol•kg−1); (e) HEPES (0.05 mol•kg−1) + NaHEPES (0.05 mol•kg−1) + NaCl (0.11 mol•kg−1); (f) HEPES (0.06 mol•kg−1) + NaHEPES (0.06 mol•kg−1) + NaCl (0.10 mol•kg−1); (g) HEPES (0.07 mol•kg−1) + NaHEPES (0.07 mol•kg−1) + NaCl (0.09 mol•kg−1); and (h) HEPES (0.08 mol•kg−1) + NaHEPES (0.08 mol•kg−1) + NaCl (0.08 mol•kg−1). Conventional paH values, for all eight buffer solutions from 5 to 55°C have been calculated. The operational pH values with liquid junction corrections, at 25 and 37°C have been determined based on the NBS/NIST standard between the physiological phosphate standard and four buffer solutions. These are recommended as pH standards for physiological fluids in the range of pH 7.3 to 7.5 at I = 0.16 mol•kg−1.
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DOI：
10.6028/jres.066a.016
发表时间：
1962-01-01
期刊：
JOURNAL OF RESEARCH OF THE NATIONAL BUREAU OF STANDARDS SECTION A-PHYSICS AND CHEMISTRY
影响因子：
--
作者：
BATES, RG
通讯作者：
BATES, RG
影响因子：
2.9
作者：
GOOD, NE;WINGET, GD;SINGH, RMM
通讯作者：
SINGH, RMM
影响因子：
1.8
作者：
Buck, RP;Rondinini, S;Wilson, GS
通讯作者：
Wilson, GS
影响因子：
1.2
作者：
Roy, RN;Mrad, DR;Wu, YC
通讯作者：
Wu, YC
影响因子：
2.9
作者：
FERGUSON, WJ;BRAUNSCHWEIGER, KI;GOOD, NE
通讯作者：
GOOD, NE