UTILLdb, a Pisum sativum in silico forward and reverse genetics tool.

UTILLdb, a Pisum sativum in silico forward and reverse genetics tool.
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DOI:
10.1186/gb-2008-9-2-r43
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发表时间:
2008
期刊:
影响因子:
12.3
通讯作者:
Bendahmane A
Bendahmane A
中科院分区:
生物学1区
文献类型:
--
作者:
Dalmais M;Schmidt J;Le Signor C;Moussy F;Burstin J;Savois V;Aubert G;Brunaud V;de Oliveira Y;Guichard C;Thompson R;Bendahmane A

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UTILLdb是来自参考豌豆EMS突变群体的突变基因的表型和序列信息的数据库。系统地表征物种中的基因功能,以农杆菌为基础的转化,如豌豆,仍然是一个挑战。为了在豌豆中开发高通量正向和反向遗传学工具,我们构建了一个参考甲基磺酸乙酯突变群体,并开发了一个数据库UTILLdb,其中包含突变基因的表型和序列信息。UTILLdb可以通过BLAST工具在线搜索TILLING等位基因,或者通过关键词搜索关于突变体的表型信息。
UTILLdb is a database of phenotypic and sequence information on mutant genes from a reference Pisum sativum EMS-mutant population. The systematic characterization of gene functions in species recalcitrant to Agrobacterium-based transformation, like Pisum sativum, remains a challenge. To develop a high throughput forward and reverse genetics tool in pea, we have constructed a reference ethylmethane sulfonate mutant population and developed a database, UTILLdb, that contains phenotypic as well as sequence information on mutant genes. UTILLdb can be searched online for TILLING alleles, through the BLAST tool, or for phenotypic information about mutants by keywords.
DOI: 10.1126/science.1086391
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