The diagnostic value of pepsin detection in saliva for gastro-esophageal reflux disease: a preliminary study from China.
The diagnostic value of pepsin detection in saliva for gastro-esophageal reflux disease: a preliminary study from China.
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DOI：
10.1186/s12876-017-0667-9
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发表时间：
2017-10-17
影响因子：
2.4
通讯作者：
Tang J
中科院分区：
文献类型：
--
作者：
Du X;Wang F;Hu Z;Wu J;Wang Z;Yan C;Zhang C;Tang J
目前没有一种诊断方法被证明是胃食管反流病（GERD）的可靠工具。唾液中的胃蛋白酶被认为是一种很有前途的胃食管反流诊断生物标志物。我们的目的是确定唾液胃蛋白酶检测对胃食管反流的诊断价值。250名有胃食管反流症状的患者和35名无症状的健康志愿者在早上醒来、午餐和晚餐后提供唾液，使用Peptest侧流装置测定胃蛋白酶。所有患者均接受24小时多通道腔内阻抗pH（24小时MII-pH）监测和上消化道内镜检查。根据24小时MII-pH值和内镜检查，将患者定义为GERD （MII-pH值异常和/或反流性食管炎），否则定义为非GERD。与非GERD患者和健康对照相比，GERD患者唾液中胃蛋白酶的流行率和胃蛋白酶浓度更高（P < 0.001）。胃蛋白酶试验诊断胃食管反流的敏感性为73%，特异性为88.3%，最佳临界值为76 ng/mL。当症状出现时收集的餐后唾液样本具有更强的识别反流的能力。唾液胃蛋白酶试验对胃反流有中等诊断价值。它具有非侵入性、操作方便、成本效益高的优点，有望取代目前的侵入性工具。ChiCTR-DDD-16009506（注册日期：2016年10月20日）
None of current diagnostic methods has been proven to be a reliable tool for gastro-esophageal reflux disease (GERD). Pepsin in saliva has been proposed as a promising diagnostic biomarker for gastro-esophageal reflux. We aimed to determine the diagnostic value of salivary pepsin detection for GERD. Two hundred and fifty patients with symptoms suggestive of GERD and 35 asymptomatic healthy volunteers provided saliva on morning waking, after lunch and dinner for pepsin determination using the Peptest lateral flow device. All patients underwent 24-h multichannel intraluminal impedance pH (24-h MII-pH) monitoring and upper gastrointestinal endoscopy. Based on 24-h MII-pH and endoscopy study, patients were defined as GERD (abnormal MII-pH results and/or reflux esophagitis) and non-GERD otherwise. Patients with GERD had a higher prevalence of pepsin in saliva and higher pepsin concentration than patients with non-GERD and healthy controls (P < 0.001 for all). The pepsin test had a sensitivity of 73% and a specificity of 88.3% for diagnosing GERD using the optimal cut-off value of 76 ng/mL. Postprandial saliva samples collected when the symptoms occurred had a more powerful ability to identify GERD. Salivary pepsin test had moderate diagnostic value for GERD. It may be a promising tool to replace the use of currently invasive tools with advantages of non-invasive, easy to perform and cost effective. ChiCTR-DDD-16009506 (date of registration: October 20, 2016).
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胃食管反流病流行病学最新进展：系统评价。
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作者：
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影响因子：
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作者：
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作者：
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影响因子：
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作者：
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DOI：
10.4291/wjgp.v7.i1.72
发表时间：
2016-02-15
期刊：
World journal of gastrointestinal pathophysiology
影响因子：
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作者：
de Bortoli, Nicola;Martinucci, Irene;Savarino, Edoardo Vincenzo
通讯作者：
Savarino, Edoardo Vincenzo