Global Sexual Fertility in the Opportunistic Pathogen Aspergillus fumigatus and Identification of New Supermater Strains.
Global Sexual Fertility in the Opportunistic Pathogen Aspergillus fumigatus and Identification of New Supermater Strains.
复制标题
DOI：
10.3390/jof6040258
复制
发表时间：
2020-10-30
期刊：
影响因子：
--
通讯作者：
Dyer PS
中科院分区：
文献类型：
--
作者：
Swilaiman SS;O'Gorman CM;Du W;Sugui JA;Del Buono J;Brock M;Kwon-Chung KJ;Szakacs G;Dyer PS
2009年首次描述了烟曲霉的性周期，分离自爱尔兰的都柏林。然而，世界范围内的分离株能够在多大程度上进行有性繁殖仍不清楚。在这项研究中，全球收集了131株交配型比例接近1：1的菌株。所有分离株都与MAT 1 -1或MAT 1 -2爱尔兰菌株杂交，来自不同大陆的分离株的子集一起杂交。发现97%的分离株与至少一个交配伴侣产生闭囊蚴，表明有性生殖不仅限于爱尔兰种群，而是全球A.烟熏。然而，大的变化被认为是在每个交叉产生的隐孢子虫的数量，表明在自然界中菌株之间的遗传交换的可能性的差异。大多数杂交产生的子囊孢子的萌发率>50%，但有很大的差异。需要高温热激来诱导子囊孢子萌发。最后，鉴定了一组新的高繁殖力的MAT 1 -1和MAT 1 -2超原种，并产生了供社区使用的嘧啶营养缺陷型。结果提供了对A.烟曲霉性周期产生遗传变异，并允许医学上重要性状的基因流动。
A sexual cycle in Aspergillus fumigatus was first described in 2009 with isolates from Dublin, Ireland. However, the extent to which worldwide isolates can undergo sexual reproduction has remained unclear. In this study a global collection of 131 isolates was established with a near 1:1 ratio of mating types. All isolates were crossed to MAT1-1 or MAT1-2 Irish strains, and a subset of isolates from different continents were crossed together. Ninety seven percent of isolates were found to produce cleistothecia with at least one mating partner, showing that sexual fertility is not limited to the Irish population but is a characteristic of global A. fumigatus. However, large variation was seen in numbers of cleistothecia produced per cross, suggesting differences in the possibility for genetic exchange between strains in nature. The majority of crosses produced ascospores with >50% germination rates, but with wide variation evident. A high temperature heat shock was required to induce ascospore germination. Finally, a new set of highly fertile MAT1-1 and MAT1-2 supermater strains were identified and pyrimidine auxotrophs generated for community use. Results provide insights into the potential for the A. fumigatus sexual cycle to generate genetic variation and allow gene flow of medically important traits.
登录
查看更多内容
影响因子：
2.9
作者：
Bertuzzi M;van Rhijn N;Krappmann S;Bowyer P;Bromley MJ;Bignell EM
通讯作者：
Bignell EM
影响因子：
--
作者：
通讯作者：
--
改进了病原曲霉曲霉中的功能分析方案。
影响因子：
4.2
作者：
He, Zhu-Mei;Price, Michael S.;OBrian, Gregory R.;Georgianna, D. Ryan;Payne, Gary A.
通讯作者：
Payne, Gary A.
影响因子：
3.5
作者：
Garcia-Rubio, Rocio;Monzon, Sara;Mellado, Emilia
通讯作者：
Mellado, Emilia
影响因子：
4.5
作者：
Fedorova ND;Khaldi N;Joardar VS;Maiti R;Amedeo P;Anderson MJ;Crabtree J;Silva JC;Badger JH;Albarraq A;Angiuoli S;Bussey H;Bowyer P;Cotty PJ;Dyer PS;Egan A;Galens K;Fraser-Liggett CM;Haas BJ;Inman JM;Kent R;Lemieux S;Malavazi I;Orvis J;Roemer T;Ronning CM;Sundaram JP;Sutton G;Turner G;Venter JC;White OR;Whitty BR;Youngman P;Wolfe KH;Goldman GH;Wortman JR;Jiang B;Denning DW;Nierman WC
通讯作者：
Nierman WC