Experimental assessment of bioenergetic differences caused by the common European mitochondrial DNA haplogroups H and T.
Experimental assessment of bioenergetic differences caused by the common European mitochondrial DNA haplogroups H and T.
复制标题
欧洲普通线粒体DNA单倍群H和T引起的生物能差异的实验评估。
DOI：
10.1016/j.gene.2008.01.007
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发表时间：
2008-03-31
期刊：
影响因子：
3.5
通讯作者：
Brand, Martin D.
中科院分区：
文献类型：
--
作者：
Amo, Taku;Yadava, Nagendra;Oh, Richard;Nicholls, David G.;Brand, Martin D.
对脓毒症后的存活率和人类精子活力的研究表明，与常见的欧洲线粒体DNA单倍型群有显著的相关性，并提出携带单倍型群H的线粒体与携带单倍型群T的线粒体具有不同的生物能量能力。然而，这种关联的有效性假设不存在核基因或其他因素的非随机影响。在这里，我们消除了核基因的任何差异的影响，通过构建transmitochondrial cybrids携带线粒体与单倍型组H或单倍型组T在培养的A549人肺癌细胞具有相同的核背景。我们比较了从这些细胞中分离的线粒体的生物能量能力和耦合效率，以及保留在细胞内的线粒体，作为这些单倍型群影响线粒体生物能量学的假设的关键实验测试。我们发现，有没有功能上重要的生物能量的差异，线粒体轴承这些单倍型群，使用分离的线粒体或线粒体内的细胞。
Studies of both survival after sepsis and sperm motility in human populations have shown significant associations with common European mitochondrial DNA haplogroups, and have led to proposals that mitochondria bearing haplogroup H have different bioenergetic capacities than those bearing haplogroup T. However, the validity of such associations assumes that there are no non-random influences of nuclear genes or other factors. Here, we removed the effect of any differences in nuclear genes by constructing transmitochondrial cybrids harbouring mitochondria with either haplogroup H or haplogroup T in cultured A549 human lung carcinoma cells with identical nuclear backgrounds. We compared the bioenergetic capacities and coupling efficiencies of mitochondria isolated from these cells, and of mitochondria retained within the cells, as a critical experimental test of the hypothesis that these haplogroups affect mitochondrial bioenergetics. We found that there were no functionally-important bioenergetic differences between mitochondria bearing these haplogroups, using either isolated mitochondria or mitochondria within cells.
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